Integrated Electrowetting Nanoinjector for Single Cell Transfection

Single cell transfection techniques are essential to understand the heterogeneity between cells. We have developed an integrated electrowetting nanoinjector (INENI) to transfect single cells. The high transfection efficiency, controlled dosage delivery and ease of INENI fabrication promote the wides...

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Bibliographic Details
Published in:Scientific reports 2016-07, Vol.6 (1), p.29051-29051, Article 29051
Main Authors: Shekaramiz, Elaheh, Varadarajalu, Ganeshkumar, Day, Philip J., Wickramasinghe, H. Kumar
Format: Article
Language:English
Subjects:
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Aqueous solutions
Efficiency
Electrodes
Electrowetting - methods
Fabrication
Heterogeneity
Humanities and Social Sciences
Humans
Microinjections - methods
multidisciplinary
Plasmids
Scanning electron microscopy
Science
Single-Cell Analysis
Transfection
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Description
Summary:Single cell transfection techniques are essential to understand the heterogeneity between cells. We have developed an integrated electrowetting nanoinjector (INENI) to transfect single cells. The high transfection efficiency, controlled dosage delivery and ease of INENI fabrication promote the widespread application of the INENI in cell transfection assays.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep29051