A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood

Research in the field of food intake regulation is gaining importance. This often includes the measurement of peptides regulating food intake. For the correct determination of a peptide's concentration, it should be stable during blood processing. However, this is not the case for several pepti...

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Bibliographic Details
Published in:Journal of visualized experiments 2016-04 (110)
Main Authors: Teuffel, Pauline, Goebel-Stengel, Miriam, Hofmann, Tobias, Prinz, Philip, Scharner, Sophie, Körner, Jan L, Grötzinger, Carsten, Rose, Matthias, Klapp, Burghard F, Stengel, Andreas
Format: Article
Language:English
Subjects:
Animals
Blood Proteins - analysis
Chemistry Techniques, Analytical - methods
Eating
Glucagon
Humans
Immunology
Insulin
Leptin
Rats
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Summary:Research in the field of food intake regulation is gaining importance. This often includes the measurement of peptides regulating food intake. For the correct determination of a peptide's concentration, it should be stable during blood processing. However, this is not the case for several peptides which are quickly degraded by endogenous peptidases. Recently, we developed a blood processing method employing Reduced temperatures, Acidification, Protease inhibition, Isotopic exogenous controls and Dilution (RAPID) for the use in rats. Here, we have established this technique for the use in humans and investigated recovery, molecular form and circulating concentration of food intake regulatory hormones. The RAPID method significantly improved the recovery for (125)I-labeled somatostatin-28 (+39%), glucagon-like peptide-1 (+35%), acyl ghrelin and glucagon (+32%), insulin and kisspeptin (+29%), nesfatin-1 (+28%), leptin (+21%) and peptide YY3-36 (+19%) compared to standard processing (EDTA blood on ice, p 
ISSN:1940-087X
1940-087X
DOI:10.3791/53959