Comparison of the Hemagglutination Inhibition Test and IgG ELISA in Categorizing Primary and Secondary Dengue Infections Based on the Plaque Reduction Neutralization Test

Secondary dengue infection by heterotypic serotypes is associated with severe manifestations of disease, that is, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The World Health Organization (WHO) has recommended criteria based on the hemagglutination inhibition (HI) test to disting...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-5
Main Authors: Williams, Maya, Widjaja, Susana, Fitri, Silvita, Parwati, Ida, Susanto, Nugroho Harry, Kosasih, Herman, Salim, Gustiani, Lukman, Nurhayati, Alisjahbana, Bachti
Format: Article
Language:English
Subjects:
Accuracy
Adolescent
Adult
Antigens
Child
Coinfection - blood
Coinfection - immunology
Coinfection - virology
Comparative analysis
Dengue - blood
Dengue - immunology
Dengue - virology
Dengue fever
Dengue hemorrhagic fever
Dengue Virus - immunology
Dengue Virus - isolation & purification
Dengue Virus - pathogenicity
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzymes
Female
Health aspects
Hemagglutination Inhibition Tests - methods
Hemolytic Plaque Technique - methods
Hospitals
Humans
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin G - immunology
Immunoglobulins
Infection
Infections
Male
Medical research
Methods
Middle Aged
Neutralization Tests - methods
Studies
Vitamin E
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Description
Summary:Secondary dengue infection by heterotypic serotypes is associated with severe manifestations of disease, that is, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The World Health Organization (WHO) has recommended criteria based on the hemagglutination inhibition (HI) test to distinguish between primary and secondary dengue infections. Since the HI test has practical limitations and disadvantages, we evaluated the accuracy of WHO HI criteria and compared it with criteria based on an IgG enzyme-linked immunosorbent assay (ELISA) using a plaque reduction neutralization test (PRNT) as the gold standard. Both WHO HI criteria and IgG ELISA criteria performed strongly (16/16) in determining primary infection. However, to determine secondary infection, the IgG ELISA criteria performed better (72/73) compared to the WHO HI criteria (23/73).
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/5253842