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Structures of Apo and Complexed Escherichia coli Glycinamide Ribonucleotide Transformylase
The three-dimensional structure of phosphoribosylglycinamide formyltransferase (10-formyltetrahydrofolate:5'-phosphoribosylglycinamide formyltransferase, EC 2.1.2.2) has been solved both as an apoenzyme at 2.8-Å resolution and as a ternary complex with the substrate glycinamide ribonucleotide a...
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Published in: | Proceedings of the National Academy of Sciences - PNAS 1992-07, Vol.89 (13), p.6114-6118 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | The three-dimensional structure of phosphoribosylglycinamide formyltransferase (10-formyltetrahydrofolate:5'-phosphoribosylglycinamide formyltransferase, EC 2.1.2.2) has been solved both as an apoenzyme at 2.8-Å resolution and as a ternary complex with the substrate glycinamide ribonucleotide and a folate inhibitor at 2.5-Å resolution. The structure is a modified doubly wound α/β sheet with flexibility in the active site, including a disordered loop in the apo structure, which is ordered in the ternary complex structure. This enzyme is a target for anti-cancer therapy and now for structure-based drug design. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.89.13.6114 |