Secretory locations of SIPC in Amphibalanus amphitrite cyprids and a novel function of SIPC in biomineralization

Settlement-inducing protein complex (SIPC) is a pheromone that triggers conspecific larval settlement in the barnacle Amphibalanus amphitrite . In the present study, immunostaining and scanning electron microscopy of SIPC revealed signals in the frontal horn pores and the secretions from carapace po...

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Bibliographic Details
Published in:Scientific reports 2016-07, Vol.6 (1), p.29376-29376, Article 29376
Main Authors: Zhang, Gen, Yang, Xiao-Xue, Leung, Pok Man, He, Li-Sheng, Chan, Tat Yin, Yan, Guo-Yong, Zhang, Yu, Sun, Jin, Xu, Ying, Qian, Pei-Yuan
Format: Article
Language:English
Subjects:
101/28
13/51
14/19
631/45/612/1231
631/601/1737
82/80
82/83
Calcite
Crystals
Environmental science
Humanities and Social Sciences
Hypotheses
Life sciences
Mineralization
Morphology
multidisciplinary
Pores
Scanning electron microscopy
Science
Science (multidisciplinary)
Shells
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Summary:Settlement-inducing protein complex (SIPC) is a pheromone that triggers conspecific larval settlement in the barnacle Amphibalanus amphitrite . In the present study, immunostaining and scanning electron microscopy of SIPC revealed signals in the frontal horn pores and the secretions from carapace pores, suggesting that SIPC might be directly secreted from these organs in A. amphitrite cyprids. Further observations showed that the frontal horn pores could contact surfaces while cyprids were “walking”. Immunostaining for SIPC on the contacted surfaces displayed SIPC signals. These signals were similar to the frontal horn pores in size and morphology, suggesting that frontal horn pores might deposit SIPC. Besides, full-length SIPC was expressed and subsequent assays indicated that recombinant SIPC was able to bind to chitins and induce the precipitation of CaCO 3 . Furthermore, recombinant SIPC inhibited the formation of vaterites and regulated the morphology of calcite crystals. The crystals that formed with recombinant SIPC were more stable against water erosion. Overall, these results reported a novel function of recombinant SIPC that regulates crystal formation in barnacle shells.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep29376