Inhibitor of p53–p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells

• Published in: Cytotechnology (Dordrecht) 2016-08, Vol.68 (4), p.1257-1265
• Main Authors: Ruan, Zhong-Bao, Zhu, Li, Yin, Yi-Gang, Chen, Ge-Cai
• Format: Article
• Language: English
• Subjects: Antibodies; Biochemistry; Biomedicine; Biotechnology; Bone marrow; Cell differentiation; Cell proliferation; Chemistry; Chemistry and Materials Science; Cyclin-dependent kinase inhibitor p21; Desmin; Flow cytometry; Immunofluorescence; Mesenchymal stem cells; Morphology; Nkx2.5 protein; Original Research; p53 Protein; Protein expression; Statistical analysis; Stem cells; Umbilical cord
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1007/s10616-015-9886-5

P53 is shown recently to play an important role in the proliferation and differentiation of mesenchymal stem cells. In this study, human umbilical cord derived mesenchymal stem cells (hUCMSCs) were isolated and purified from the umbilical cords of normal or cesarean term deliveries, after treatment with 20 μmol/L PFT-α for 24 h, hUCMSCs were continued to be cultured for 4 weeks, cardiac-specific protein expression of cTnI, Desmin and Nkx2.5 was determined using immunofluorescence assay and RT-PCR. The expression of p53 and p21 was detected by western blot. Results showed that no expression of cTnI, Desmin or Nkx2.5 was observed in the control and the PFT-α group at 1 week after induction. However, after 4 weeks, while control group still had little expression of cTnI, Desmin and Nkx2.5, the PFT-α group demonstrated strong expression of cTnI, Desmin and Nkx2.5 ( P