Microbiological assay for vitamin B12 performed in 96-well microtitre plates

A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plates using a colistin sulphate resistant strain of Lactobacillus leichmannii (NCIB 12519), and cryopreserved cultures is described. The new assay correlated well with a more conventional "tube" assay a...

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Bibliographic Details
Published in:Journal of clinical pathology 1991-07, Vol.44 (7), p.592-595
Main Authors: Kelleher, B P, Broin, S D
Format: Article
Language:English
Subjects:
beta-Lactamases - administration & dosage
Biological and medical sciences
Biological Assay - methods
Humans
Investigative techniques, diagnostic techniques (general aspects)
Lactobacillus - growth & development
Lactobacillus - metabolism
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Vitamin B 12 - blood
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Summary:A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plates using a colistin sulphate resistant strain of Lactobacillus leichmannii (NCIB 12519), and cryopreserved cultures is described. The new assay correlated well with a more conventional "tube" assay and was not influenced by the presence of antibiotics in serum. Evaluation of assay performance showed excellent interassay and intra-assay precision with quantitative recovery of added cyanocobalamin over a wide range of additions (94.9%-102.1%). The advantages of short incubation time, easy reading, and minimal reagent costs make this assay an attractive option in the routine clinical laboratory and in research.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.44.7.592