Measurement of alkaline phosphatase of intestinal origin in plasma by p-bromotetramisole inhibition

L-p-bromotetramisole was used to inhibit non-intestinal alkaline phosphatase (of liver or bone origin) (EC 3.1.3.1; ALP) in plasma, and intestinal ALP was measured from the uninhibited activity. The method of determination is convenient and correlated well with measurement by immunocapture assay. If...

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Bibliographic Details
Published in:Journal of clinical pathology 1991-03, Vol.44 (3), p.236-237
Main Authors: Kuwana, T, Rosalki, S B
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - antagonists & inhibitors
Alkaline Phosphatase - blood
Antibodies, Monoclonal
Biological and medical sciences
Bone and Bones - enzymology
Female
Humans
Intestines - enzymology
Investigative techniques, diagnostic techniques (general aspects)
Levamisole - analogs & derivatives
Liver - enzymology
Male
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Tetramisole - analogs & derivatives
Tetramisole - pharmacology
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Summary:L-p-bromotetramisole was used to inhibit non-intestinal alkaline phosphatase (of liver or bone origin) (EC 3.1.3.1; ALP) in plasma, and intestinal ALP was measured from the uninhibited activity. The method of determination is convenient and correlated well with measurement by immunocapture assay. If carried out in parallel with wheat-germ lectin precipitation of bone ALP, subtraction of intestinal ALP activity from that of non-bone ALP in the supernatant can be used to measure the ALP that originates from the liver in men and non-pregnant women.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.44.3.236