Nanostructured lipid carriers loaded with resveratrol modulate human dendritic cells

Dendritic cells (DCs) are promising targets for drug delivery, as they can induce immunity or tolerance. The current study aims to examine the potential of using nanostructured lipid carriers (NLC) as delivery systems for human DC by evaluating nanoparticle internalization, cell labeling, and drug a...

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Bibliographic Details
Published in:International journal of nanomedicine 2016-01, Vol.11, p.3501-3516
Main Authors: Barbosa, João P, Neves, Ana R, Silva, Andreia M, Barbosa, Mário A, Reis, M Salette, Santos, Susana G
Format: Article
Language:English
Subjects:
Antigens
Antigens, CD - metabolism
Arthritis
Blood & organ donations
CD83 Antigen
Cell Differentiation - drug effects
Cytokines
Dendritic cells
Dendritic Cells - drug effects
Dendritic Cells - metabolism
Drug Carriers - administration & dosage
Drug Carriers - chemistry
Drug Carriers - pharmacology
Drug delivery systems
Drugs
Flow Cytometry
Fluorescein
Fluorescein-5-isothiocyanate - chemistry
Humans
Immune Tolerance - drug effects
Immunoglobulins - metabolism
Interleukin-12 - metabolism
Lipids
Lipids - chemistry
Lipids - pharmacology
Membrane Glycoproteins - metabolism
Monocytes - metabolism
Nanoparticles
Nanostructures - administration & dosage
Nanostructures - chemistry
NF-kappa B - metabolism
Original Research
Resveratrol
Scanning electron microscopy
Stilbenes - administration & dosage
Stilbenes - pharmacology
Tumor necrosis factor
Tumor Necrosis Factor-alpha - metabolism
Tumor necrosis factor-TNF
Vehicles
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Summary:Dendritic cells (DCs) are promising targets for drug delivery, as they can induce immunity or tolerance. The current study aims to examine the potential of using nanostructured lipid carriers (NLC) as delivery systems for human DC by evaluating nanoparticle internalization, cell labeling, and drug activity. NLC were formulated incorporating the fluorochrome fluorescein isothiocyanate (FITC-NLC) or the natural anti-inflammatory molecule resveratrol (rsv-NLC). Primary human DCs were differentiated from peripheral blood monocytes, and the innovative imaging flow cytometry technique was used to examine FITC-NLC internalization. The capacity of rsv-NLC to inhibit DC activation in response to proinflammatory cytokine tumor necrosis factor-α (TNF- α) was investigated by conventional flow cytometry. A combination of imaging and conventional flow cytometry was used to assess NLC cytotoxicity. The results obtained indicate that both NLC formulations were stable over time, with mean diameter
ISSN:1178-2013
1176-9114
1178-2013
DOI:10.2147/IJN.S108694