Growth of Neisseria gonorrhoeae in brain heart infusion

Although relatively complex liquid media are available for growing N. gonorrhoeae a simply prepared, heat stable liquid medium would be welcomed by venereologists for a variety of general purposes. Attention was recently drawn to a simple semi-solid medium consisting of brain heart infusion, agar an...

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Bibliographic Details
Published in:Journal of clinical pathology 1982-01, Vol.35 (1), p.119-119
Main Authors: Annear, D I, Wild, B
Format: Article
Language:English
Subjects:
Culture Media
Neisseria gonorrhoeae
Neisseria gonorrhoeae - growth & development
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Summary:Although relatively complex liquid media are available for growing N. gonorrhoeae a simply prepared, heat stable liquid medium would be welcomed by venereologists for a variety of general purposes. Attention was recently drawn to a simple semi-solid medium consisting of brain heart infusion, agar and starch which supported growth of all 65 isolates of gonococci tested. Although this medium was very useful, its agar content was a disadvantage for some procedures such as those requiring centrifuged deposits of cells. Further work showed that gonococci grew well in unmodified Brain Heart Infusion (BHI) (Difco) but that the physical conditions under which the organisms were grown greatly influenced the yield.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.35.1.119