Oral exposure to environmental pollutant benzo[a]pyrene impacts the intestinal epithelium and induces gut microbial shifts in murine model

Gut microbiota dysbiosis are associated with a wide range of human diseases, including inflammatory bowel diseases. The physiopathology of these diseases has multifactorial aetiology in which environmental factors, particularly pollution could play a crucial role. Among the different pollutants list...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-08, Vol.6 (1), p.31027-11, Article 31027
Main Authors: Ribière, Céline, Peyret, Pierre, Parisot, Nicolas, Darcha, Claude, Déchelotte, Pierre J., Barnich, Nicolas, Peyretaillade, Eric, Boucher, Delphine
Format: Article
Language:English
Subjects:
14/63
631/326/2565/2142
631/326/2565/855
64/60
Abundance
Animal models
Benzo(a)pyrene
Digestive system
Dysbacteriosis
Environmental factors
Epithelium
Exposure
Gastrointestinal tract
Humanities and Social Sciences
Inflammatory bowel diseases
Intestinal microflora
Intestine
Life Sciences
Mucosa
multidisciplinary
Pollutants
Polycyclic aromatic hydrocarbons
Pyrene
Rodents
rRNA 16S
Science
Science (multidisciplinary)
Small intestine
Toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Gut microbiota dysbiosis are associated with a wide range of human diseases, including inflammatory bowel diseases. The physiopathology of these diseases has multifactorial aetiology in which environmental factors, particularly pollution could play a crucial role. Among the different pollutants listed, Polycyclic Aromatic Hydrocarbons (PAHs) are subject to increased monitoring due to their wide distribution and high toxicity on Humans. Here, we used 16S rRNA gene sequencing to investigate the impact of benzo[a]pyrene (BaP, most toxic PAH) oral exposure on the faecal and intestinal mucosa-associated bacteria in C57BL/6 mice. Intestinal inflammation was also evaluated by histological observations. BaP oral exposure significantly altered the composition and the abundance of the gut microbiota and led to moderate inflammation in ileal and colonic mucosa. More severe lesions were observed in ileal segment. Shifts in gut microbiota associated with moderate inflammatory signs in intestinal mucosa would suggest the establishment of a pro-inflammatory intestinal environment following BaP oral exposure. Therefore, under conditions of genetic susceptibility and in association with other environmental factors, exposure to this pollutant could trigger and/or accelerate the development of inflammatory pathologies.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep31027