Report of a joint DMRQC/Organon field trial to detect hepatitis A IgM by ELISA

The results of a field trial of a joint DMRQC/Organon ELISA kit for the detection of hepatitis A IgM antibody are reported. The participating laboratories were asked to use the kit to test a panel of 360 specimens consisting of duplicate coded samples of 180 sera. The panel was also tested by MACRIA...

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Published in:Journal of clinical pathology 1983-10, Vol.36 (10), p.1111-1115
Main Authors: Supran, E M, Craske, J, Hart, R J, Kurtz, J B, Parry, J V, Skidmore, S J, Gardner, P S
Format: Article
Language:English
Subjects:
Acute Disease
Antibodies, Viral - analysis
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay
Evaluation Studies as Topic
Hepatitis A - diagnosis
hepatitis A virus
Hepatovirus - immunology
Human viral diseases
Humans
Immunoenzyme Techniques
Immunoglobulin M - analysis
Infectious diseases
Medical sciences
Radioimmunoassay
Reagent Kits, Diagnostic
Viral diseases
Viral hepatitis
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cited_by cdi_FETCH-LOGICAL-b544t-5a520bae5e984f38a472b3f13c9935a96cb36d568c564283f34b62afc08d40d73
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container_issue 10
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container_title Journal of clinical pathology
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creator Supran, E M
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Gardner, P S
description The results of a field trial of a joint DMRQC/Organon ELISA kit for the detection of hepatitis A IgM antibody are reported. The participating laboratories were asked to use the kit to test a panel of 360 specimens consisting of duplicate coded samples of 180 sera. The panel was also tested by MACRIA in the Virus Reference Laboratory, Colindale. The ELISA was shown to be specific and sensitive giving good discrimination between acute and late convalescent hepatitis A sera. It was proposed that the same cut-off control as is used in the RIA (equivalent to 10 RIA units) should be adopted for the ELISA also.
doi_str_mv 10.1136/jcp.36.10.1111
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subjects Acute Disease
Antibodies, Viral - analysis
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay
Evaluation Studies as Topic
Hepatitis A - diagnosis
hepatitis A virus
Hepatovirus - immunology
Human viral diseases
Humans
Immunoenzyme Techniques
Immunoglobulin M - analysis
Infectious diseases
Medical sciences
Radioimmunoassay
Reagent Kits, Diagnostic
Viral diseases
Viral hepatitis
title Report of a joint DMRQC/Organon field trial to detect hepatitis A IgM by ELISA
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