Translocation frequency of double-stranded DNA through a solid-state nanopore

Solid-state nanopores are single-molecule sensors that measure changes in ionic current as charged polymers such as DNA pass through. Here, we present comprehensive experiments on the length, voltage, and salt dependence of the frequency of double-stranded DNA translocations through conical quartz n...

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Bibliographic Details
Published in:Physical review. E 2016-02, Vol.93 (2), p.022401-022401, Article 022401
Main Authors: Bell, Nicholas A W, Muthukumar, Murugappan, Keyser, Ulrich F
Format: Article
Language:English
Subjects:
Base Pairing
DNA - chemistry
DNA - metabolism
Movement
Nanopores
Potassium Chloride - chemistry
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Summary:Solid-state nanopores are single-molecule sensors that measure changes in ionic current as charged polymers such as DNA pass through. Here, we present comprehensive experiments on the length, voltage, and salt dependence of the frequency of double-stranded DNA translocations through conical quartz nanopores with mean opening diameter 15 nm. We observe an entropic barrier-limited, length-dependent translocation frequency at 4M LiCl salt concentration and a drift-dominated, length-independent translocation frequency at 1M KCl salt concentration. These observations are described by a unifying convection-diffusion equation, which includes the contribution of an entropic barrier for polymer entry.
ISSN:2470-0045
2470-0053
DOI:10.1103/PhysRevE.93.022401