Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico

Nucleoprotein (N) gene from rabies virus (RABV) is a useful sequence target for variant studies. Several specific RABV variants have been characterized in different mammalian hosts such as skunk, dog, and bats by using anti-nucleocapsid monoclonal antibodies (MAbs) via indirect fluorescent antibody...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-15
Main Authors: Lara-Padilla, Eleazar, Chavira-Suárez, Erika, Ramírez-Hernández, Dolores G., Fernando, Bastida-González, Paola, Zárate-Segura
Format: Article
Language:English
Subjects:
Animals
Bats
Brain
Design
Disease
DNA Primers - genetics
Dogs
Genes
Genotyping Techniques - methods
Laboratories
Mexico
Monoclonal antibodies
Mortality
Nucleocapsid Proteins - genetics
Rabies
Rabies virus
Rabies virus - genetics
Real-Time Polymerase Chain Reaction - methods
Studies
Surveillance
Viruses
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Summary:Nucleoprotein (N) gene from rabies virus (RABV) is a useful sequence target for variant studies. Several specific RABV variants have been characterized in different mammalian hosts such as skunk, dog, and bats by using anti-nucleocapsid monoclonal antibodies (MAbs) via indirect fluorescent antibody (IFA) test, a technique not available in many laboratories in Mexico. In the present study, a total of 158 sequences of N gene from RABV were used to design eight pairs of primers (four external and four internal primers), for typing four different RABV variants (dog, skunk, vampire bat, and nonhematophagous bat) which are most common in Mexico. The results indicate that the primer and the typing variant from the brain samples, submitted to nested and/or real-time PCR, are in agreement in all four singleplex reactions, and the designed primer pairs are an alternative for use in specific variant RABV typing.
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/4659470