SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley

A high-density linkage map is a valuable tool for functional genomics and breeding. A newly developed sequence-based marker technology, restriction site associated DNA (RAD) sequencing, has been proven to be powerful for the rapid discovery and genotyping of genome-wide single nucleotide polymorphis...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2016-08, Vol.6 (1), p.31741-31741, Article 31741
Main Authors: Ren, Xifeng, Wang, Jibin, Liu, Lipan, Sun, Genlou, Li, Chengdao, Luo, Hong, Sun, Dongfa
Format: Article
Language:English
Subjects:
45
45/23
631/208/8
631/337/103/90
631/449/711
9/30
Barley
Chromosome 7
Chromosomes
Cloning
Deoxyribonucleic acid
DNA
DNA sequencing
Gene mapping
Gene polymorphism
Genetic distance
Genomics
Genotyping
Humanities and Social Sciences
multidisciplinary
Nucleotide sequence
Plant breeding
Science
Single-nucleotide polymorphism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A high-density linkage map is a valuable tool for functional genomics and breeding. A newly developed sequence-based marker technology, restriction site associated DNA (RAD) sequencing, has been proven to be powerful for the rapid discovery and genotyping of genome-wide single nucleotide polymorphism (SNP) markers and for the high-density genetic map construction. The objective of this research was to construct a high-density genetic map of barley using RAD sequencing. 1894 high-quality SNP markers were developed and mapped onto all seven chromosomes together with 68 SSR markers. These 1962 markers constituted a total genetic length of 1375.8 cM and an average of 0.7 cM between adjacent loci. The number of markers within each linkage group ranged from 209 to 396. The new recessive dwarfing gene btwd1 in Huaai 11 was mapped onto the high density linkage maps. The result showed that the btwd1 is positioned between SNP marks 7HL_6335336 and 7_249275418 with a genetic distance of 0.9 cM and 0.7 cM on chromosome 7H, respectively. The SNP-based high-density genetic map developed and the dwarfing gene btwd1 mapped in this study provide critical information for position cloning of the btwd1 gene and molecular breeding of barley.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep31741