Rapid processing of fetal skin for prenatal diagnosis by light and electron microscopy

A method has been developed for rapid processing of fetal skin for prenatal diagnosis of hereditary skin diseases by light and electron microscopy. Fixation, dehydration, embedding, and polymerisation can be achieved in about 5 h. The quality of tissue preservation compares favourably with that prod...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical pathology 1984-06, Vol.37 (6), p.633-638
Main Authors: Eady, R A, Gunner, D B, Tidman, M J, Nicolaides, K H, Rodeck, C H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Dermatology
Female
Fetal Diseases - diagnosis
Fetal Diseases - pathology
Fetoscopy
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Microscopy
Microscopy, Electron
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Pregnancy
Pregnancy Trimester, Second
Prenatal Diagnosis - methods
Skin - pathology
Skin Diseases - diagnosis
Skin Diseases - genetics
Skin Diseases - pathology
Time Factors
Tissue Preservation - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-b505t-ee779efd5ff0dfdaab16a1570d3344cbdde621cc31bcad6d46a95380a10b66663
cites cdi_FETCH-LOGICAL-b505t-ee779efd5ff0dfdaab16a1570d3344cbdde621cc31bcad6d46a95380a10b66663
container_end_page 638
container_issue 6
container_start_page 633
container_title Journal of clinical pathology
container_volume 37
creator Eady, R A
Gunner, D B
Tidman, M J
Nicolaides, K H
Rodeck, C H
description A method has been developed for rapid processing of fetal skin for prenatal diagnosis of hereditary skin diseases by light and electron microscopy. Fixation, dehydration, embedding, and polymerisation can be achieved in about 5 h. The quality of tissue preservation compares favourably with that produced by slower conventional techniques. This procedure may provoke a wider interest in the potential use of fetal skin biopsy in prenatal diagnosis, especially if identification of structural abnormalities is a feasible alternative to more time consuming biochemical analysis.
doi_str_mv 10.1136/jcp.37.6.633
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_498836</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3971188401</sourcerecordid><originalsourceid>FETCH-LOGICAL-b505t-ee779efd5ff0dfdaab16a1570d3344cbdde621cc31bcad6d46a95380a10b66663</originalsourceid><addsrcrecordid>eNp9kc-LEzEUx4Moa129eRUCil6cmkwyyczBgxR3V1gVRIu38CY_uulOk9lkKva_N7WlqAffJfC-n_f4vnwRekrJnFIm3qz1OGdyLuaCsXtoRrmsK065uI9mhNS06iQXD9GjnNeEUCYpO0NnQtaNoHSGll9g9AaPKWqbsw8rHB12doIB51sfsIupiDbAvmM8rELMPuN-hwe_upkwBIPtYPWUYsAbr1PMOo67x-iBgyHbJ8f3HH27eP91cVVdf778sHh3XfUNaabKWik760zjHDHOAPRUAG0kMYxxrntjrKip1oz2GowwXEDXsJYAJb0oxc7R28PecdtvrNE2TAkGNSa_gbRTEbz6Wwn-Rq3iD8W7tmX7-ZfH-RTvtjZPauOztsMAwcZtVi0lUnS8K-Dzf8B13KZQblNUSsJr0f628_pA7f8hJ-tOTihR-7BUCUsxqYQqYRX82Z_uT_AxnaK_OOqQNQwuQdA-n7C27WjXNgWrDpjPk_15kiHdlk1MNurTcqE-ym75_fJiqa4K_-rA95v1_w3-Ahg9us0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770426866</pqid></control><display><type>article</type><title>Rapid processing of fetal skin for prenatal diagnosis by light and electron microscopy</title><source>PubMed Central</source><creator>Eady, R A ; Gunner, D B ; Tidman, M J ; Nicolaides, K H ; Rodeck, C H</creator><creatorcontrib>Eady, R A ; Gunner, D B ; Tidman, M J ; Nicolaides, K H ; Rodeck, C H</creatorcontrib><description>A method has been developed for rapid processing of fetal skin for prenatal diagnosis of hereditary skin diseases by light and electron microscopy. Fixation, dehydration, embedding, and polymerisation can be achieved in about 5 h. The quality of tissue preservation compares favourably with that produced by slower conventional techniques. This procedure may provoke a wider interest in the potential use of fetal skin biopsy in prenatal diagnosis, especially if identification of structural abnormalities is a feasible alternative to more time consuming biochemical analysis.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.37.6.633</identifier><identifier>PMID: 6725611</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Biological and medical sciences ; Dermatology ; Female ; Fetal Diseases - diagnosis ; Fetal Diseases - pathology ; Fetoscopy ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Microscopy ; Microscopy, Electron ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Pregnancy ; Pregnancy Trimester, Second ; Prenatal Diagnosis - methods ; Skin - pathology ; Skin Diseases - diagnosis ; Skin Diseases - genetics ; Skin Diseases - pathology ; Time Factors ; Tissue Preservation - methods</subject><ispartof>Journal of clinical pathology, 1984-06, Vol.37 (6), p.633-638</ispartof><rights>1985 INIST-CNRS</rights><rights>Copyright BMJ Publishing Group LTD Jun 1984</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b505t-ee779efd5ff0dfdaab16a1570d3344cbdde621cc31bcad6d46a95380a10b66663</citedby><cites>FETCH-LOGICAL-b505t-ee779efd5ff0dfdaab16a1570d3344cbdde621cc31bcad6d46a95380a10b66663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC498836/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC498836/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27915,27916,53782,53784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8891985$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6725611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eady, R A</creatorcontrib><creatorcontrib>Gunner, D B</creatorcontrib><creatorcontrib>Tidman, M J</creatorcontrib><creatorcontrib>Nicolaides, K H</creatorcontrib><creatorcontrib>Rodeck, C H</creatorcontrib><title>Rapid processing of fetal skin for prenatal diagnosis by light and electron microscopy</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>A method has been developed for rapid processing of fetal skin for prenatal diagnosis of hereditary skin diseases by light and electron microscopy. Fixation, dehydration, embedding, and polymerisation can be achieved in about 5 h. The quality of tissue preservation compares favourably with that produced by slower conventional techniques. This procedure may provoke a wider interest in the potential use of fetal skin biopsy in prenatal diagnosis, especially if identification of structural abnormalities is a feasible alternative to more time consuming biochemical analysis.</description><subject>Biological and medical sciences</subject><subject>Dermatology</subject><subject>Female</subject><subject>Fetal Diseases - diagnosis</subject><subject>Fetal Diseases - pathology</subject><subject>Fetoscopy</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Microscopy</subject><subject>Microscopy, Electron</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, Second</subject><subject>Prenatal Diagnosis - methods</subject><subject>Skin - pathology</subject><subject>Skin Diseases - diagnosis</subject><subject>Skin Diseases - genetics</subject><subject>Skin Diseases - pathology</subject><subject>Time Factors</subject><subject>Tissue Preservation - methods</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNp9kc-LEzEUx4Moa129eRUCil6cmkwyyczBgxR3V1gVRIu38CY_uulOk9lkKva_N7WlqAffJfC-n_f4vnwRekrJnFIm3qz1OGdyLuaCsXtoRrmsK065uI9mhNS06iQXD9GjnNeEUCYpO0NnQtaNoHSGll9g9AaPKWqbsw8rHB12doIB51sfsIupiDbAvmM8rELMPuN-hwe_upkwBIPtYPWUYsAbr1PMOo67x-iBgyHbJ8f3HH27eP91cVVdf778sHh3XfUNaabKWik760zjHDHOAPRUAG0kMYxxrntjrKip1oz2GowwXEDXsJYAJb0oxc7R28PecdtvrNE2TAkGNSa_gbRTEbz6Wwn-Rq3iD8W7tmX7-ZfH-RTvtjZPauOztsMAwcZtVi0lUnS8K-Dzf8B13KZQblNUSsJr0f628_pA7f8hJ-tOTihR-7BUCUsxqYQqYRX82Z_uT_AxnaK_OOqQNQwuQdA-n7C27WjXNgWrDpjPk_15kiHdlk1MNurTcqE-ym75_fJiqa4K_-rA95v1_w3-Ahg9us0</recordid><startdate>19840601</startdate><enddate>19840601</enddate><creator>Eady, R A</creator><creator>Gunner, D B</creator><creator>Tidman, M J</creator><creator>Nicolaides, K H</creator><creator>Rodeck, C H</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19840601</creationdate><title>Rapid processing of fetal skin for prenatal diagnosis by light and electron microscopy</title><author>Eady, R A ; Gunner, D B ; Tidman, M J ; Nicolaides, K H ; Rodeck, C H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b505t-ee779efd5ff0dfdaab16a1570d3344cbdde621cc31bcad6d46a95380a10b66663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Biological and medical sciences</topic><topic>Dermatology</topic><topic>Female</topic><topic>Fetal Diseases - diagnosis</topic><topic>Fetal Diseases - pathology</topic><topic>Fetoscopy</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Microscopy</topic><topic>Microscopy, Electron</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, Second</topic><topic>Prenatal Diagnosis - methods</topic><topic>Skin - pathology</topic><topic>Skin Diseases - diagnosis</topic><topic>Skin Diseases - genetics</topic><topic>Skin Diseases - pathology</topic><topic>Time Factors</topic><topic>Tissue Preservation - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eady, R A</creatorcontrib><creatorcontrib>Gunner, D B</creatorcontrib><creatorcontrib>Tidman, M J</creatorcontrib><creatorcontrib>Nicolaides, K H</creatorcontrib><creatorcontrib>Rodeck, C H</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eady, R A</au><au>Gunner, D B</au><au>Tidman, M J</au><au>Nicolaides, K H</au><au>Rodeck, C H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid processing of fetal skin for prenatal diagnosis by light and electron microscopy</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>1984-06-01</date><risdate>1984</risdate><volume>37</volume><issue>6</issue><spage>633</spage><epage>638</epage><pages>633-638</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>A method has been developed for rapid processing of fetal skin for prenatal diagnosis of hereditary skin diseases by light and electron microscopy. Fixation, dehydration, embedding, and polymerisation can be achieved in about 5 h. The quality of tissue preservation compares favourably with that produced by slower conventional techniques. This procedure may provoke a wider interest in the potential use of fetal skin biopsy in prenatal diagnosis, especially if identification of structural abnormalities is a feasible alternative to more time consuming biochemical analysis.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>6725611</pmid><doi>10.1136/jcp.37.6.633</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0021-9746
ispartof Journal of clinical pathology, 1984-06, Vol.37 (6), p.633-638
issn 0021-9746
1472-4146
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_498836
source PubMed Central
subjects Biological and medical sciences
Dermatology
Female
Fetal Diseases - diagnosis
Fetal Diseases - pathology
Fetoscopy
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Microscopy
Microscopy, Electron
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Pregnancy
Pregnancy Trimester, Second
Prenatal Diagnosis - methods
Skin - pathology
Skin Diseases - diagnosis
Skin Diseases - genetics
Skin Diseases - pathology
Time Factors
Tissue Preservation - methods
title Rapid processing of fetal skin for prenatal diagnosis by light and electron microscopy
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T06%3A01%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20processing%20of%20fetal%20skin%20for%20prenatal%20diagnosis%20by%20light%20and%20electron%20microscopy&rft.jtitle=Journal%20of%20clinical%20pathology&rft.au=Eady,%20R%20A&rft.date=1984-06-01&rft.volume=37&rft.issue=6&rft.spage=633&rft.epage=638&rft.pages=633-638&rft.issn=0021-9746&rft.eissn=1472-4146&rft.coden=JCPAAK&rft_id=info:doi/10.1136/jcp.37.6.633&rft_dat=%3Cproquest_pubme%3E3971188401%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-b505t-ee779efd5ff0dfdaab16a1570d3344cbdde621cc31bcad6d46a95380a10b66663%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1770426866&rft_id=info:pmid/6725611&rfr_iscdi=true