A plasmonic colorimetric strategy for visual miRNA detection based on hybridization chain reaction

In this work, a novel colorimetric strategy for miRNA analysis is proposed based on hybridization chain reaction (HCR)-mediated localized surface plasmon resonance (LSPR) variation of silver nanoparticles (AgNPs). miRNA in the sample to be tested is able to release HCR initiator from a solid interfa...

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Bibliographic Details
Published in:Scientific reports 2016-08, Vol.6 (1), p.32219, Article 32219
Main Authors: Miao, Jie, Wang, Jingsheng, Guo, Jinyang, Gao, Huiguang, Han, Kun, Jiang, Chengmin, Miao, Peng
Format: Article
Language:English
Subjects:
631/1647/2196/2197
639/638/11/872
Biological samples
Colorimetry
Deoxyribonucleic acid
DNA
Enzymes
Humanities and Social Sciences
Hybridization
Influenza
miRNA
multidisciplinary
Nanoparticles
Science
Silver
Surface plasmon resonance
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Summary:In this work, a novel colorimetric strategy for miRNA analysis is proposed based on hybridization chain reaction (HCR)-mediated localized surface plasmon resonance (LSPR) variation of silver nanoparticles (AgNPs). miRNA in the sample to be tested is able to release HCR initiator from a solid interface to AgNPs colloid system by toehold exchange-mediated strand displacement, which then triggers the consumption of fuel strands with single-stranded tails for HCR. The final produced long nicked double-stranded DNA loses the ability to protect AgNPs from salt-induced aggregation. The stability variation of the colloid system can then be monitored by recording corresponding UV-vis spectrum and initial miRNA level is thus determined. This sensing system involves only four DNA strands which is quite simple. The practical utility is confirmed to be excellent by employing different biological samples.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep32219