Nitrification and its influence on biogeochemical cycles from the equatorial Pacific to the Arctic Ocean

• Published in: The ISME Journal 2016-09, Vol.10 (9), p.2184-2197
• Main Authors: Shiozaki, Takuhei, Ijichi, Minoru, Isobe, Kazuo, Hashihama, Fuminori, Nakamura, Ken-ichi, Ehama, Makoto, Hayashizaki, Ken-ichi, Takahashi, Kazutaka, Hamasaki, Koji, Furuya, Ken
• Format: Article
• Language: English
• Subjects: 38/22; 38/77; 38/90; 631/158/47; 631/158/852; 704/172; Ammonia; Ammonia - metabolism; Ammonium; Archaea; Archaea - genetics; Archaea - metabolism; Arctic Regions; Biogeochemical cycles; Biomedical and Life Sciences; Cold regions; Ecology; Euphotic zone; Evolutionary Biology; Green belts; Life Sciences; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Nitrification; Nitrogen Cycle; Nutrient availability; Organic matter; Original; original-article; Oxidation; Oxidation-Reduction; Pacific Ocean; Primary production
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/ismej.2016.18

We examined nitrification in the euphotic zone, its impact on the nitrogen cycles, and the controlling factors along a 7500 km transect from the equatorial Pacific Ocean to the Arctic Ocean. Ammonia oxidation occurred in the euphotic zone at most of the stations. The gene and transcript abundances for ammonia oxidation indicated that the shallow clade archaea were the major ammonia oxidizers throughout the study regions. Ammonia oxidation accounted for up to 87.4% (average 55.6%) of the rate of nitrate assimilation in the subtropical oligotrophic region. However, in the shallow Bering and Chukchi sea shelves (bottom ⩽67 m), the percentage was small (0–4.74%) because ammonia oxidation and the abundance of ammonia oxidizers were low, the light environment being one possible explanation for the low activity. With the exception of the shallow bottom stations, depth-integrated ammonia oxidation was positively correlated with depth-integrated primary production. Ammonia oxidation was low in the high-nutrient low-chlorophyll subarctic region and high in the Bering Sea Green Belt, and primary production in both was influenced by micronutrient supply. An ammonium kinetics experiment demonstrated that ammonia oxidation did not increase significantly with the addition of 31–1560 n m ammonium at most stations except in the Bering Sea Green Belt. Thus, the relationship between ammonia oxidation and primary production does not simply indicate that ammonia oxidation increased with ammonium supply through decomposition of organic matter produced by primary production but that ammonia oxidation might also be controlled by micronutrient availability as with primary production.