High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster

• Published in: Oxidative medicine and cellular longevity 2016-01, Vol.2016 (2016), p.1-12
• Main Authors: Posser, Thaís, Prigol, Marina, Franco, Jeferson Luis, Wallau, Gabriel L., Zemolin, A. P. P., Barreto Meichtry, Luana, Cardoso Bortolotto, Vandreza, Machado Araujo, Stífani, Poetini Silva, Márcia Rósula, Trindade de Paula, Mariane, Jesse, Cristiano Ricardo
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase - metabolism; Adipocytes; Analysis; Animals; Antioxidants; Antioxidants - metabolism; Biomarkers - metabolism; Blood Glucose - metabolism; Body Weight - drug effects; Coconut Oil; Diet; Diet, High-Fat; Drosophila; Drosophila melanogaster - drug effects; Drosophila melanogaster - genetics; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Enzymes; Fatty acids; Gene expression; Gene Expression Regulation - drug effects; Genetic aspects; Heat shock proteins; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Insects; Kinases; Lipid peroxidation; Lipids; Longevity - drug effects; Longevity - genetics; Metabolism; Metabolites; Mitochondria - drug effects; Mitochondria - metabolism; Mitogen-Activated Protein Kinases - genetics; Mitogen-Activated Protein Kinases - metabolism; Motor Activity - drug effects; Nutrition research; Obesity; Oxidative stress; Oxidative Stress - drug effects; Plant Oils - pharmacology; Real-Time Polymerase Chain Reaction; Saturated fatty acids; Studies; Survival Rate; Triglycerides; Triglycerides - metabolism
• ISSN: 1942-0900; 1942-0994
• DOI: 10.1155/2016/4018157

The consumption of a high-fat diet (HFD) causes alteration in normal metabolism affecting lifespan of flies; however molecular mechanism associated with this damage in flies is not well known. This study evaluates the effects of ingestion of a diet supplemented with 10% and 20% of coconut oil, which is rich in saturated fatty acids, on oxidative stress and cells stress signaling pathways. After exposure to the diet for seven days, cellular and mitochondrial viability, lipid peroxidation and antioxidant enzymes SOD and CAT activity, and mRNA expression of antioxidant enzymes HSP83 and MPK2 were analyzed. To confirm the damage effect of diet on flies, survival and lifespan were investigated. The results revealed that the HFD augmented the rate of lipid peroxidation and SOD and CAT activity and induced a higher expression of HSP83 and MPK2 mRNA. In parallel, levels of enzymes involved in lipid metabolism (ACSL1 and ACeCS1) were increased. Our data demonstrate that association among metabolic changes, oxidative stress, and protein signalization might be involved in shortening the lifespan of flies fed with a HFD.