Improved method for isolation and growth of Chlamydia trachomatis in McCoy cells treated with cycloheximide using polyethylene glycol

The sensitivity of non-replicating McCoy cells pretreated with polyethylene glycol for the isolation of Chlamydia trachomatis from clinical specimens and for the growth of a laboratory strain was compared with the sensitivity of untreated non-replicating cell cultures. The concentration of polyethyl...

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Bibliographic Details
Published in:Journal of clinical pathology 1985-09, Vol.38 (9), p.1052-1054
Main Authors: Mohammed, N R, Hillary, I B
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriological Techniques
Bacteriology
Barbital - pharmacology
Biological and medical sciences
Cells, Cultured
Cervix Uteri - microbiology
Chlamydia trachomatis - isolation & purification
Culture Media
Cycloheximide - pharmacology
Dose-Response Relationship, Drug
Female
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Polyethylene Glycols - pharmacology
Time Factors
Urethra - microbiology
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Summary:The sensitivity of non-replicating McCoy cells pretreated with polyethylene glycol for the isolation of Chlamydia trachomatis from clinical specimens and for the growth of a laboratory strain was compared with the sensitivity of untreated non-replicating cell cultures. The concentration of polyethylene glycol in different solutions and the time of addition to the cell culture medium were critical. A concentration of 35% polyethylene glycol in barbitone added to the cell culture growth medium either immediately before or immediately after infection with chlamydia increased the number of inclusions detected. The rate of isolation obtained from clinical specimens was also increased when cell cultures treated with polyethylene glycol were used.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.38.9.1052