Stenotrophomonas sp. RZS 7, a novel PHB degrader isolated from plastic contaminated soil in Shahada, Maharashtra, Western India

This paper reports an isolation and identification of novel poly-β-hydroxybutyrate (PHB) degrading bacterium Stenotrophomonas sp. RZS 7 and studies on its extracellular PHB degrading depolymerase enzyme. The bacterium isolated from soil samples of plastic contaminated sites of municipal area in Shah...

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Bibliographic Details
Published in:3 Biotech 2016-12, Vol.6 (2), p.179-5, Article 179
Main Authors: Wani, S. J., Shaikh, S. S., Tabassum, B., Thakur, R., Gulati, A., Sayyed, R. Z.
Format: Article
Language:English
Subjects:
Agriculture
Bacteria
Biodegradation
Bioinformatics
Biomaterials
Biotechnology
Cancer Research
Carbon
Chemistry
Chemistry and Materials Science
Enzyme kinetics
Fatty acids
Microorganisms
Short Reports
Soil contamination
Stem Cells
Stenotrophomonas
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Summary:This paper reports an isolation and identification of novel poly-β-hydroxybutyrate (PHB) degrading bacterium Stenotrophomonas sp. RZS 7 and studies on its extracellular PHB degrading depolymerase enzyme. The bacterium isolated from soil samples of plastic contaminated sites of municipal area in Shahada, Maharashtra, Western India. It was identified as Stenotrophomonas sp. RZS 7 based on polyphasic approach. The bacterium grew well in minimal salt medium (MSM) and produced a zone (4.2 mm) of PHB hydrolysis on MSM containing PHB as the only source of nutrient. An optimum yield of enzyme was obtained on the fifth day of incubation at 37 °C and at pH 6.0. Further increase in enzyme production was recorded with Ca 2+ ions, while other metal ions like Fe 2+ (1 mM) and chemical viz. mercaptoethanol severally affected the production of enzyme.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-016-0477-8