In Vitro Antimicrobial Bioassays, DPPH Radical Scavenging Activity, and FTIR Spectroscopy Analysis of Heliotropium bacciferum

• Published in: BioMed research international 2016-01, Vol.2016 (2016), p.1-12
• Main Authors: Ahmad, Sohail, Akbar, Riaz Ullah Said, Abd El-Salam, Nasser M.
• Format: Article
• Language: English
• Subjects: Alcohol; Amines; Analysis; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - chemistry; Antibiotics; Antimicrobial agents; Antioxidants; Bacteria; Bacterial Physiological Phenomena - drug effects; Bioassays; Biological Assay - methods; Biological products; Biphenyl Compounds - chemistry; Biphenyl Compounds - isolation & purification; Cell Survival - drug effects; Cell Survival - physiology; Chromatography; Diarrhea; Drug resistance in microorganisms; Drugs; E coli; Flavonoids; Flowers & plants; Free Radical Scavengers - chemistry; Free Radicals - chemistry; Free Radicals - isolation & purification; Heliotropium; Heliotropium - chemistry; Heliotropium - classification; Herbal medicine; Infections; Infrared spectroscopy; Medicine, Botanic; Medicine, Herbal; Microorganisms; Mortality; Olefins; Organic compounds; Phytochemicals; Picrates - chemistry; Picrates - isolation & purification; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Plants, Medicinal - chemistry; Plants, Medicinal - classification; Species Specificity; Spectroscopy, Fourier Transform Infrared - methods; Studies
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2016/3818945

The present study deals with the antimicrobial, antioxidant, and functional group analysis of Heliotropium bacciferum extracts. Disc diffusion susceptibility method was followed for antimicrobial assessment. Noteworthy antimicrobial activities were recorded by various plant extracts against antibiotic resistant microorganisms. Plant flower extracts antioxidant activity was investigated against 2, 2-diphenyl-1-picryl hydrazyl radical by ultraviolet spectrophotometer (517 nm). Plant extracts displayed noteworthy radical scavenging activities at all concentrations (25–225 μg/mL). Notable activities were recorded by crude, chloroform and ethyl acetate extracts up to 88.27% at 225 μg/mL concentration. Compounds functional groups were examined by Fourier transform infrared spectroscopic studies. Alkanes, alkenes, alkyl halides, amines, carboxylic acids, amides, esters, alcohols, phenols, nitrocompounds, and aromatic compounds were identified by FTIR analysis. Thin layer chromatography bioautography was carried out for all plant extracts. Different bands were separated by various solvent systems. The results of the current study justify the use of Heliotropium bacciferum in traditional remedial herbal medicines.