PAQR3 gene expression and its methylation level in colorectal cancer tissues

• Published in: Oncology letters 2016-09, Vol.12 (3), p.1773-1778
• Main Authors: Li, Ri-Heng, Zhang, Ai-Min, Li, Shuang, Li, Tian-Yang, Wang, Lian-Jing, Zhang, Hao-Ran, Shi, Jian-Wei, Liu, Xiao-Rui, Chen, Yuan, Chen, Ya-Chao, Wei, Teng-Yao, Gao, Ying, Li, Wei, Tang, Hong-Ying, Tang, Mei-Yu
• Format: Article
• Language: English
• Subjects: Age; Breast cancer; Cell growth; Colorectal cancer; Development and progression; Gastric cancer; Gene expression; Genetic aspects; Health aspects; Medical diagnosis; Membrane proteins; Metabolism; Metastasis; methylation; mRNA; Oncology; PAQR3; Phenols; Phosphorylation; Properties; Proteins; Signal transduction; Surgery; western blot analysis
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2016.4843

The aim of the present study was to investigate the PAQR3 gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively. MSP was used to detect the methylation level of PAQR3 gene in colorectal samples, which was compared with colorectal data. The results showed that a decreased expression level of PAQR3 mRNA in colorectal cancer tissues and the expression reduction rate was 57.4% (31/54). Similarly, the expression level of PAQR3 protein was reduced in cancer tissues, and the reduction rate was 46.3% (25/54), while the protein expression reduction rate in cancer adjacent tissue was 5.6% (3/54), and the difference was statistically significant (P