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Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration
Drought stress is one of the most adverse environmental constraints to plant growth and productivity. Comparative proteomics of drought-tolerant and sensitive wheat genotypes is a strategy to understand the complexity of molecular mechanism of wheat in response to drought. This study attempted to ex...
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| Published in: | BMC plant biology 2016-08, Vol.16 (1), p.188-188, Article 188 |
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| creator | Cheng, Lixiang Wang, Yuping He, Qiang Li, Huijun Zhang, Xiaojing Zhang, Feng |
| description | Drought stress is one of the most adverse environmental constraints to plant growth and productivity. Comparative proteomics of drought-tolerant and sensitive wheat genotypes is a strategy to understand the complexity of molecular mechanism of wheat in response to drought. This study attempted to extend findings regarding the potential proteomic dynamics in wheat under drought stress and to enrich the research content of drought tolerance mechanism.
A comparative proteomics approach was applied to analyze proteome change of Xihan No. 2 (a drought-tolerant cultivar) and Longchun 23 (a drought-sensitive cultivar) subjected to a range of dehydration treatments (18 h, 24 h and 48 h) and rehydration treatment (R24 h) using 2-DE, respectively. Quantitative image analysis showed a total of 172 protein spots in Xihan No. 2 and 215 spots from Longchun 23 with their abundance significantly altered (p |
| doi_str_mv | 10.1186/s12870-016-0871-8 |
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A comparative proteomics approach was applied to analyze proteome change of Xihan No. 2 (a drought-tolerant cultivar) and Longchun 23 (a drought-sensitive cultivar) subjected to a range of dehydration treatments (18 h, 24 h and 48 h) and rehydration treatment (R24 h) using 2-DE, respectively. Quantitative image analysis showed a total of 172 protein spots in Xihan No. 2 and 215 spots from Longchun 23 with their abundance significantly altered (p < 0.05) more than 2.5-fold. Out of these spots, a total of 84 and 64 differentially abundant proteins were identified by MALDI-TOF/TOF MS in Xihan No. 2 and Longchun 23, respectively. Most of these identified proteins were involved in metabolism, photosynthesis, defence and protein translation/processing/degradation in both two cultivars. In addition, the proteins involved in redox homeostasis, energy, transcription, cellular structure, signalling and transport were also identified. Furthermore, the comparative analysis of drought-responsive proteome allowed for the general elucidation of the major mechanisms associated with differential responses to drought of both two cultivars. These cellular processes work more cooperatively to re-establish homeostasis in Xihan No. 2 than Longchun 23. The resistance mechanisms of Xihan No. 2 mainly included changes in the metabolism of carbohydrates and amino acids as well as in the activation of more antioxidation and defense systems and in the levels of proteins involved in ATP synthesis and protein degradation/refolding.
This study revealed that the levels of a number of proteins involved in various cellular processes were affected by drought stress in two wheat cultivars with different drought tolerance. The results showed that there exist specific responses to drought in Xihan No. 2 and Longchun 23. The proposed hypothetical model would explain the interaction of these identified proteins that are associated with drought-responses in two cultivars, and help in developing strategies to improve drought tolerance in wheat.</description><identifier>ISSN: 1471-2229</identifier><identifier>EISSN: 1471-2229</identifier><identifier>DOI: 10.1186/s12870-016-0871-8</identifier><identifier>PMID: 27576435</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Droughts ; Electrophoresis, Gel, Two-Dimensional ; Environmental aspects ; Gene Expression Regulation, Plant ; Genetic aspects ; Hardiness ; Photosynthesis ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants ; Proteome - chemistry ; Proteome - genetics ; Proteome - metabolism ; Proteomics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Triticum - chemistry ; Triticum - genetics ; Triticum - metabolism ; Water - analysis ; Water - metabolism ; Wheat</subject><ispartof>BMC plant biology, 2016-08, Vol.16 (1), p.188-188, Article 188</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>The Author(s). 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-e3486a7d44c6261d3c7007fb6ef408f21eaead9c7b46bebc92cd88f37960c6ec3</citedby><cites>FETCH-LOGICAL-c566t-e3486a7d44c6261d3c7007fb6ef408f21eaead9c7b46bebc92cd88f37960c6ec3</cites><orcidid>0000-0003-4387-1212</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006382/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006382/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,37012,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27576435$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Lixiang</creatorcontrib><creatorcontrib>Wang, Yuping</creatorcontrib><creatorcontrib>He, Qiang</creatorcontrib><creatorcontrib>Li, Huijun</creatorcontrib><creatorcontrib>Zhang, Xiaojing</creatorcontrib><creatorcontrib>Zhang, Feng</creatorcontrib><title>Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration</title><title>BMC plant biology</title><addtitle>BMC Plant Biol</addtitle><description>Drought stress is one of the most adverse environmental constraints to plant growth and productivity. Comparative proteomics of drought-tolerant and sensitive wheat genotypes is a strategy to understand the complexity of molecular mechanism of wheat in response to drought. This study attempted to extend findings regarding the potential proteomic dynamics in wheat under drought stress and to enrich the research content of drought tolerance mechanism.
A comparative proteomics approach was applied to analyze proteome change of Xihan No. 2 (a drought-tolerant cultivar) and Longchun 23 (a drought-sensitive cultivar) subjected to a range of dehydration treatments (18 h, 24 h and 48 h) and rehydration treatment (R24 h) using 2-DE, respectively. Quantitative image analysis showed a total of 172 protein spots in Xihan No. 2 and 215 spots from Longchun 23 with their abundance significantly altered (p < 0.05) more than 2.5-fold. Out of these spots, a total of 84 and 64 differentially abundant proteins were identified by MALDI-TOF/TOF MS in Xihan No. 2 and Longchun 23, respectively. Most of these identified proteins were involved in metabolism, photosynthesis, defence and protein translation/processing/degradation in both two cultivars. In addition, the proteins involved in redox homeostasis, energy, transcription, cellular structure, signalling and transport were also identified. Furthermore, the comparative analysis of drought-responsive proteome allowed for the general elucidation of the major mechanisms associated with differential responses to drought of both two cultivars. These cellular processes work more cooperatively to re-establish homeostasis in Xihan No. 2 than Longchun 23. The resistance mechanisms of Xihan No. 2 mainly included changes in the metabolism of carbohydrates and amino acids as well as in the activation of more antioxidation and defense systems and in the levels of proteins involved in ATP synthesis and protein degradation/refolding.
This study revealed that the levels of a number of proteins involved in various cellular processes were affected by drought stress in two wheat cultivars with different drought tolerance. The results showed that there exist specific responses to drought in Xihan No. 2 and Longchun 23. The proposed hypothetical model would explain the interaction of these identified proteins that are associated with drought-responses in two cultivars, and help in developing strategies to improve drought tolerance in wheat.</description><subject>Droughts</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Environmental aspects</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genetic aspects</subject><subject>Hardiness</subject><subject>Photosynthesis</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants</subject><subject>Proteome - chemistry</subject><subject>Proteome - genetics</subject><subject>Proteome - metabolism</subject><subject>Proteomics</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Triticum - chemistry</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Water - analysis</subject><subject>Water - metabolism</subject><subject>Wheat</subject><issn>1471-2229</issn><issn>1471-2229</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNptks1q3DAUhU1padK0D9BNEXSTLDyV_CPJm0IY-hMYKLTpWmik67GKLU0lOcm8Tp-0d5h0yEDxwlfyd450fU9RvGV0wZjkHxKrpKAlZbykUrBSPivOWYNFVVXd8yf1WfEqpV-UMiGb7mVxVolW8KZuz4s_yzBtddTZ3QHZxpAhTM4k4sZxThn3IZE8ADGIjfDg8o6EntgY5s2QSYTkUtbeAJnADNq7NKHWk3wfyP0AOpPL2-iyM_NENCQ8BYvV4oqYecSFjonM3kIkFoad3V8jeKK9Refj-nXxotdjgjeP74vi5-dPt8uv5erbl5vl9ao0Lee5hLqRXAvbNIZXnNnaCEpFv-bQN1T2FQMN2nZGrBu-hrXpKmOl7GvRcWo4mPqi-Hjw3c7rCawBj_2PahvdpONOBe3U6RfvBrUJd6qllNeyQoPLR4MYfs_YrZpcMjCO2kOYk2KStZ1ocXKIvj-gGz2Ccr4P6Gj2uLpuuJQ4HbqnFv-h8LGAQwoeeof7J4KrEwEyGR7yRs8pqZsf309ZdmBNDClF6I-dMqr28VKHeCmMl9rHS0nUvHv6i46Kf3mq_wKFk8_D</recordid><startdate>20160831</startdate><enddate>20160831</enddate><creator>Cheng, Lixiang</creator><creator>Wang, Yuping</creator><creator>He, Qiang</creator><creator>Li, Huijun</creator><creator>Zhang, Xiaojing</creator><creator>Zhang, Feng</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4387-1212</orcidid></search><sort><creationdate>20160831</creationdate><title>Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration</title><author>Cheng, Lixiang ; Wang, Yuping ; He, Qiang ; Li, Huijun ; Zhang, Xiaojing ; Zhang, Feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-e3486a7d44c6261d3c7007fb6ef408f21eaead9c7b46bebc92cd88f37960c6ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Droughts</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Environmental aspects</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genetic aspects</topic><topic>Hardiness</topic><topic>Photosynthesis</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants</topic><topic>Proteome - chemistry</topic><topic>Proteome - genetics</topic><topic>Proteome - metabolism</topic><topic>Proteomics</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Triticum - chemistry</topic><topic>Triticum - genetics</topic><topic>Triticum - metabolism</topic><topic>Water - analysis</topic><topic>Water - metabolism</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Lixiang</creatorcontrib><creatorcontrib>Wang, Yuping</creatorcontrib><creatorcontrib>He, Qiang</creatorcontrib><creatorcontrib>Li, Huijun</creatorcontrib><creatorcontrib>Zhang, Xiaojing</creatorcontrib><creatorcontrib>Zhang, Feng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC plant biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Lixiang</au><au>Wang, Yuping</au><au>He, Qiang</au><au>Li, Huijun</au><au>Zhang, Xiaojing</au><au>Zhang, Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration</atitle><jtitle>BMC plant biology</jtitle><addtitle>BMC Plant Biol</addtitle><date>2016-08-31</date><risdate>2016</risdate><volume>16</volume><issue>1</issue><spage>188</spage><epage>188</epage><pages>188-188</pages><artnum>188</artnum><issn>1471-2229</issn><eissn>1471-2229</eissn><abstract>Drought stress is one of the most adverse environmental constraints to plant growth and productivity. Comparative proteomics of drought-tolerant and sensitive wheat genotypes is a strategy to understand the complexity of molecular mechanism of wheat in response to drought. This study attempted to extend findings regarding the potential proteomic dynamics in wheat under drought stress and to enrich the research content of drought tolerance mechanism.
A comparative proteomics approach was applied to analyze proteome change of Xihan No. 2 (a drought-tolerant cultivar) and Longchun 23 (a drought-sensitive cultivar) subjected to a range of dehydration treatments (18 h, 24 h and 48 h) and rehydration treatment (R24 h) using 2-DE, respectively. Quantitative image analysis showed a total of 172 protein spots in Xihan No. 2 and 215 spots from Longchun 23 with their abundance significantly altered (p < 0.05) more than 2.5-fold. Out of these spots, a total of 84 and 64 differentially abundant proteins were identified by MALDI-TOF/TOF MS in Xihan No. 2 and Longchun 23, respectively. Most of these identified proteins were involved in metabolism, photosynthesis, defence and protein translation/processing/degradation in both two cultivars. In addition, the proteins involved in redox homeostasis, energy, transcription, cellular structure, signalling and transport were also identified. Furthermore, the comparative analysis of drought-responsive proteome allowed for the general elucidation of the major mechanisms associated with differential responses to drought of both two cultivars. These cellular processes work more cooperatively to re-establish homeostasis in Xihan No. 2 than Longchun 23. The resistance mechanisms of Xihan No. 2 mainly included changes in the metabolism of carbohydrates and amino acids as well as in the activation of more antioxidation and defense systems and in the levels of proteins involved in ATP synthesis and protein degradation/refolding.
This study revealed that the levels of a number of proteins involved in various cellular processes were affected by drought stress in two wheat cultivars with different drought tolerance. The results showed that there exist specific responses to drought in Xihan No. 2 and Longchun 23. The proposed hypothetical model would explain the interaction of these identified proteins that are associated with drought-responses in two cultivars, and help in developing strategies to improve drought tolerance in wheat.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>27576435</pmid><doi>10.1186/s12870-016-0871-8</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-4387-1212</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Droughts Electrophoresis, Gel, Two-Dimensional Environmental aspects Gene Expression Regulation, Plant Genetic aspects Hardiness Photosynthesis Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plants Proteome - chemistry Proteome - genetics Proteome - metabolism Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Triticum - chemistry Triticum - genetics Triticum - metabolism Water - analysis Water - metabolism Wheat |
| title | Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration |
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