Mass Spectrometric Collisional Activation and Product Ion Mobility of Human Serum Neutral Lipid Extracts

A novel method for lipid analysis called CTS (collisional activation and traveling wave mass spectrometry), involving tandem mass spectrometry of all precursor ions with ion mobility determinations of all product ions, was applied to a sample of human serum. The resulting four-dimensional data set (...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-06, Vol.88 (12), p.6274-6282
Main Authors: Hankin, Joseph A, Barkley, Robert M, Zemski-Berry, Karin, Deng, Yiming, Murphy, Robert C
Format: Article
Language:English
Subjects:
Activation analysis
Analytical chemistry
Data analysis
Data processing
Fatty Acids, Unsaturated - blood
Fatty Acids, Unsaturated - chemistry
Humans
Ion mobility
Ionic mobility
Ions
Ions - chemistry
Lipids
Lipids - blood
Mass spectrometry
Molecules
Precursors
Serums
Spectrometry, Mass, Electrospray Ionization - methods
Triglycerides - blood
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Description
Summary:A novel method for lipid analysis called CTS (collisional activation and traveling wave mass spectrometry), involving tandem mass spectrometry of all precursor ions with ion mobility determinations of all product ions, was applied to a sample of human serum. The resulting four-dimensional data set (precursor ion, product ion, ion mobility value, and intensity) was found to be useful for characterization of lipids as classes as well as for identification of specific species. Utilization of ion mobility measurements of the product ions is a novel approach for lipid analysis. The trends and patterns of product mobility values when visually displayed yield information on lipid classes and specific species independent of mass determination. Collection of a comprehensive set of data that incorporates all precursor–product relationships, combined with ion mobility measurements of all products, enables data analysis where different molecular properties can be juxtaposed and analyzed to assist with class and species identification. Overall, CTS is a powerful, specific, and comprehensive method for lipid analysis.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b00292