A single amino acid mutation of OsSBEIIb contributes to resistant starch accumulation in rice

Foods rich in resistant starch can help prevent various diseases, including diabetes, colon cancers, diarrhea, and chronic renal and hepatic diseases. Variations in starch biosynthesis enzymes could contribute to the high content of resistant starch in some cultivars of rice (Oryza sativa L.). Our p...

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Bibliographic Details
Published in:Breeding Science 2016, Vol.66(4), pp.481-489
Main Authors: Yang, Ruifang, Bai, Jianjiang, Fang, Jun, Wang, Ying, Lee, Gangseob, Piao, Zhongze
Format: Article
Language:English
Subjects:
Alleles
Amino acids
Amylopectin
Biosynthesis
Complementation
complementation assay
Cultivars
Diabetes mellitus
Diarrhea
Endosperm
Enzymes
Fatty acids
Flowering
Food
Jiangtangdao1
Mutation
Oryza sativa
Physicochemical properties
Research Paper
resistant starch
Rice
sbe3-rs
Seeds
Starch
starch branching enzyme
Starch-branching enzymes
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Summary:Foods rich in resistant starch can help prevent various diseases, including diabetes, colon cancers, diarrhea, and chronic renal and hepatic diseases. Variations in starch biosynthesis enzymes could contribute to the high content of resistant starch in some cultivars of rice (Oryza sativa L.). Our previously published work indicated that the sbe3-rs gene in the rice mutant line, ‘Jiangtangdao1’ was a putative allele of the rice starch branching enzyme gene SBEIIb (previously known as SBE3); sbe3-rs might control the biosynthesis of the high resistant starch content in the rice line. Biomolecular analysis showed that the activity of SBEs was significantly lower in soluble extracts of immature seeds harvested from ‘Jiangtangdao1’ 15 days after flowering than in the extracts of the wild-type rice line ‘Huaqingdao’. We performed gene complementation assays by introducing the wild-type OsSBEIIb into the sbe3-rs mutant ‘Jiangtangdao1’. The genetically complemented lines demonstrated restored seed-related traits. The structures of endosperm amylopectin and the morphological and physicochemical properties of the starch granules in the transformants recovered to wild-type levels. This study provides evidence that sbe3-rs is a novel allele of OsSBEIIb, responsible for biosynthesis of high resistant starch in ‘Jiangtangdao1’.
ISSN:1344-7610
1347-3735
DOI:10.1270/jsbbs.16037