Humanized Monoclonal Antibody That Passively Protects Mice against Systemic and Intranasal Ricin Toxin Challenge

PB10 is a murine monoclonal antibody against an immunodominant epitope on ricin toxin's enzymatic subunit. Here, we characterize a fully humanized version of PB10 IgG1 (hPB10) and demonstrate that it has potent in vitro and in vivo toxin-neutralizing activities. We also report the minimum serum...

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Bibliographic Details
Published in:Clinical and vaccine immunology 2016-09, Vol.23 (9), p.795-799
Main Authors: Van Slyke, Greta, Sully, Erin K, Bohorova, Natasha, Bohorov, Ognian, Kim, Do, Pauly, Michael H, Whaley, Kevin J, Zeitlin, Larry, Mantis, Nicholas J
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - administration & dosage
Antibodies, Monoclonal - isolation & purification
Antibodies, Monoclonal - pharmacology
Antibodies, Monoclonal, Humanized - administration & dosage
Antibodies, Monoclonal, Humanized - isolation & purification
Antibodies, Monoclonal, Humanized - pharmacology
Antitoxins - administration & dosage
Antitoxins - isolation & purification
Antitoxins - pharmacology
Chemical Warfare Agents - toxicity
Disease Models, Animal
Female
Mice, Inbred BALB C
Poisoning - therapy
Ricin - toxicity
Survival Analysis
Treatment Outcome
Vaccines
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Description
Summary:PB10 is a murine monoclonal antibody against an immunodominant epitope on ricin toxin's enzymatic subunit. Here, we characterize a fully humanized version of PB10 IgG1 (hPB10) and demonstrate that it has potent in vitro and in vivo toxin-neutralizing activities. We also report the minimum serum concentrations of hPB10 required to protect mice against 10 times the 50% lethal dose of ricin when delivered by injection and inhalation.
ISSN:1556-6811
1556-679X
DOI:10.1128/CVI.00088-16