CAT7 and cat7l Long Non-coding RNAs Tune Polycomb Repressive Complex 1 Function during Human and Zebrafish Development

The essential functions of polycomb repressive complex 1 (PRC1) in development and gene silencing are thought to involve long non-coding RNAs (lncRNAs), but few specific lncRNAs that guide PRC1 activity are known. We screened for lncRNAs, which co-precipitate with PRC1 from chromatin and found candi...

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Bibliographic Details
Published in:The Journal of biological chemistry 2016-09, Vol.291 (37), p.19558-19572
Main Authors: Ray, Mridula K., Wiskow, Ole, King, Matthew J., Ismail, Nidha, Ergun, Ayla, Wang, Yanqun, Plys, Aaron J., Davis, Christopher P., Kathrein, Katie, Sadreyev, Ruslan, Borowsky, Mark L., Eggan, Kevin, Zon, Leonard, Galloway, Jenna L., Kingston, Robert E.
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation - physiology
chromatin
chromatin regulation
CsCl density gradient
development
gene expression
Gene Expression Regulation, Developmental - physiology
Gene Regulation
HeLa Cells
Humans
long noncoding RNA (long ncRNA, lncRNA)
mammal
Mice
Models, Biological
Neurons - metabolism
polycomb
Polycomb Repressive Complex 1 - genetics
Polycomb Repressive Complex 1 - metabolism
PRC1
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
zebrafish
Zebrafish - embryology
Zebrafish - genetics
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
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Summary:The essential functions of polycomb repressive complex 1 (PRC1) in development and gene silencing are thought to involve long non-coding RNAs (lncRNAs), but few specific lncRNAs that guide PRC1 activity are known. We screened for lncRNAs, which co-precipitate with PRC1 from chromatin and found candidates that impact polycomb group protein (PcG)-regulated gene expression in vivo. A novel lncRNA from this screen, CAT7, regulates expression and polycomb group binding at the MNX1 locus during early neuronal differentiation. CAT7 contains a unique tandem repeat domain that shares high sequence similarity to a non-syntenic zebrafish analog, cat7l. Defects caused by interference of cat7l RNA during zebrafish embryogenesis were rescued by human CAT7 RNA, enhanced by interference of a PRC1 component, and suppressed by interference of a known PRC1 target gene, demonstrating cat7l genetically interacts with a PRC1. We propose a model whereby PRC1 acts in concert with specific lncRNAs and that CAT7/cat7l represents convergent lncRNAs that independently evolved to tune PRC1 repression at individual loci.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M116.730853