Double-layered cell transfer technology for bone regeneration

• Published in: Scientific reports 2016-09, Vol.6 (1), p.33286-33286, Article 33286
• Main Authors: Akazawa, Keiko, Iwasaki, Kengo, Nagata, Mizuki, Yokoyama, Naoki, Ayame, Hirohito, Yamaki, Kazumasa, Tanaka, Yuichi, Honda, Izumi, Morioka, Chikako, Kimura, Tsuyoshi, Komaki, Motohiro, Kishida, Akio, Izumi, Yuichi, Morita, Ikuo
• Format: Article
• Language: English
• Subjects: 631/532/2074; 631/61/2035; Adherent cells; Amnion - transplantation; Amniotic membrane; Animals; Bone growth; Bone Regeneration; Cell Differentiation - genetics; Cell growth; Consent; Humanities and Social Sciences; Humans; Ligaments; Localization; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells - cytology; Mesenchyme; Methods; Mice; multidisciplinary; Osteoblasts; Osteoblasts - transplantation; Osteogenesis; Periodontal Ligament - cytology; Periodontal Ligament - transplantation; Periodontics; R&D; Regeneration; Research & development; Science; Stem cells; Tissue engineering; Tissue Engineering - methods; Tissue Scaffolds; Topology; Transplantation
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep33286

For cell-based medicine, to mimic in vivo cellular localization, various tissue engineering approaches have been studied to obtain a desirable arrangement of cells on scaffold materials. We have developed a novel method of cell manipulation called “cell transfer technology”, enabling the transfer of cultured cells onto scaffold materials, and controlling cell topology. Here we show that using this technique, two different cell types can be transferred onto a scaffold surface as stable double layers or in patterned arrangements. Various combinations of adherent cells were transferred to a scaffold, amniotic membrane, in overlapping bilayers (double-layered cell transfer), and transferred cells showed stability upon deformations of the material including folding and trimming. Transplantation of mesenchymal stem cells from periodontal ligaments (PDLSC) and osteoblasts, using double-layered cell transfer significantly enhanced bone formation, when compared to single cell type transplantation. Our findings suggest that this double-layer cell transfer is useful to produce a cell transplantation material that can bear two cell layers. Moreover, the transplantation of an amniotic membrane with PDLSCs/osteoblasts by cell transfer technology has therapeutic potential for bone defects. We conclude that cell transfer technology provides a novel and unique cell transplantation method for bone regeneration.