Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome

Background Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs. Aim Determine whether routinely used plasma centrifugation protocols (1500× g 10 min; 3000× g 5 min) influence non-targeted metabolomic analyses. Methods Nuclear magne...

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Bibliographic Details
Published in:Metabolomics 2016-10, Vol.12 (10), p.159-159, Article 159
Main Authors: Lesche, Dorothea, Geyer, Roland, Lienhard, Daniel, Nakas, Christos T., Diserens, Gaëlle, Vermathen, Peter, Leichtle, Alexander B.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Developmental Biology
Life Sciences
Molecular Medicine
Short Communication
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Summary:Background Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs. Aim Determine whether routinely used plasma centrifugation protocols (1500× g 10 min; 3000× g 5 min) influence non-targeted metabolomic analyses. Methods Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements. Results Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols. Conclusion Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.
ISSN:1573-3882
1573-3890
DOI:10.1007/s11306-016-1109-3