Imaging brain activity during seizures in freely behaving rats using a miniature multi-modal imaging system

We report on a miniature label-free imaging system for monitoring brain blood flow and blood oxygenation changes in awake, freely behaving rats. The device, weighing 15 grams, enables imaging in a ∼ 2 × 2 mm field of view with 4.4 μm lateral resolution and 1 - 8 Hz temporal sampling rate. The imagin...

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Bibliographic Details
Published in:Biomedical optics express 2016-09, Vol.7 (9), p.3596-3609
Main Authors: Sigal, Iliya, Koletar, Margaret M, Ringuette, Dene, Gad, Raanan, Jeffrey, Melanie, Carlen, Peter L, Stefanovic, Bojana, Levi, Ofer
Format: Article
Language:English
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Summary:We report on a miniature label-free imaging system for monitoring brain blood flow and blood oxygenation changes in awake, freely behaving rats. The device, weighing 15 grams, enables imaging in a ∼ 2 × 2 mm field of view with 4.4 μm lateral resolution and 1 - 8 Hz temporal sampling rate. The imaging is performed through a chronically-implanted cranial window that remains optically clear between 2 to > 6 weeks after the craniotomy. This imaging method is well suited for longitudinal studies of chronic models of brain diseases and disorders. In this work, it is applied to monitoring neurovascular coupling during drug-induced absence-like seizures 6 weeks following the craniotomy.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.7.003596