Rigidity of silicone substrates controls cell spreading and stem cell differentiation

The dependences of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on the rigidity and porosity of the substrates have recently been a subject of some controversy. In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard sili...

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Bibliographic Details
Published in:Scientific reports 2016-09, Vol.6 (1), p.33411-33411, Article 33411
Main Authors: Vertelov, Grigory, Gutierrez, Edgar, Lee, Sin-Ae, Ronan, Edward, Groisman, Alex, Tkachenko, Eugene
Format: Article
Language:English
Subjects:
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Adipogenesis - drug effects
Cell Culture Techniques
Cell differentiation
Cell Differentiation - drug effects
Cell Proliferation - drug effects
Cell spreading
Gels
Humanities and Social Sciences
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology
Hydrogels
Mesenchymal Stem Cells - drug effects
Mesenchyme
multidisciplinary
Osteogenesis - drug effects
Osteogenesis - genetics
Porosity
Rigidity
Science
Silicones
Silicones - pharmacology
Stem cells
Substrates
Topography
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Summary:The dependences of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on the rigidity and porosity of the substrates have recently been a subject of some controversy. In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard silicone gels we show that harder gels are more osteogenic, softer gels are more adipogenic, and cell spreading areas increase with the silicone gel substrate rigidity. The results of our study indicate that substrate rigidity induces some universal cellular responses independently of the porosity or topography of the substrate.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep33411