Hepatocellular carcinoma stem cell-like cells are enriched following low-dose 5-fluorouracil chemotherapy

It has been proposed that cancer stem cells (CSCs) are involved in tumor resistance to chemotherapy and tumor relapse. The goal of the present study was to determine the effect of low-dose 5-fluorouracil (5-Fu) on enriched hepatocellular CSC-like cells. Increased cell motility and epithelial-mesench...

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Bibliographic Details
Published in:Oncology letters 2016-10, Vol.12 (4), p.2511-2516
Main Authors: Zhan, Yongqiang, Mou, Lisha, Cheng, Kangwen, Wang, Chengyou, Deng, Xuesong, Chen, Junren, Fan, Zhibing, Ni, Yong
Format: Article
Language:English
Subjects:
5-fluorouracil
Cancer cells
cancer stem cell-like cells
Cancer therapies
Cell cycle
Chemotherapy
Development and progression
Drug dosages
Drug therapy
Flow cytometry
Fluorouracil
Growth
hepatocellular carcinoma
Hepatoma
Liver cancer
Metastasis
Oncology
Patient outcomes
Penicillin
Proteins
Stem cells
tumor
Tumorigenesis
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Summary:It has been proposed that cancer stem cells (CSCs) are involved in tumor resistance to chemotherapy and tumor relapse. The goal of the present study was to determine the effect of low-dose 5-fluorouracil (5-Fu) on enriched hepatocellular CSC-like cells. Increased cell motility and epithelial-mesenchymal transition were observed by migration assay in human hepatoblastoma PLC/RAF/5 cells following 5-Fu treatment, as well as a significant enhancement in their sphere-forming abilities. CSC-like cells were identified by side population cell analysis. The percentage of CSC-like cells in the surviving cells was greatly increased in response to 5-Fu. These findings indicate that low-dose 5-Fu treatment may efficiently enrich the CSC-like cell population in PLC/RAF/5 cells.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2016.4975