Partially Purified Extracts of Sea Anemone Anemonia viridis Affect the Growth and Viability of Selected Tumour Cell Lines

In the last few years, marine species have been investigated for the presence of natural products with anticancer activity. Using reversed phase chromatography, low molecular weight proteins were fractionated from the sea anemone Anemonia viridis. Four different fractions were evaluated for their cy...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-12
Main Authors: Mazzola, Salvatore, Colombo, Paolo, Cuttitta, Angela, Tagliavia, Marcello, Salamone, Monica, Bonura, Angela, Bennici, Carmelo, Vlah, Sara, Masullo, Tiziana, Longo, Alessandra, Bulati, Matteo, Nicosia, Aldo
Format: Article
Language:English
Subjects:
Anemonia viridis
Animals
Antineoplastic Agents - administration & dosage
Antineoplastic Agents - chemical synthesis
Antineoplastic Agents - isolation & purification
Apoptosis
Cancer
Cell Extracts - administration & dosage
Cell Extracts - isolation & purification
Cell growth
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
Cells
Cytotoxicity
Dose-Response Relationship, Drug
Humans
Morphology
Neoplasms, Experimental - drug therapy
Neoplasms, Experimental - pathology
Peptides
Proteins
Sea Anemones - chemistry
Treatment Outcome
Tumors
Venom
Water quality
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the last few years, marine species have been investigated for the presence of natural products with anticancer activity. Using reversed phase chromatography, low molecular weight proteins were fractionated from the sea anemone Anemonia viridis. Four different fractions were evaluated for their cytotoxic activity by means of erythrocyte haemolysis test, MTS, and LDH assays. Finally, the antiproliferative activities of three of these fractions were studied on PC3, PLC/PRF/5, and A375 human cancer cell lines. Our analysis revealed that the four fractions showed different protein contents and diverse patterns of activity towards human PBMC and cancer cell lines. Interestingly, fractions III and IV exerted cytotoxic effects on human cells. Conversely, fractions I and II displayed very low toxic effects associated with antiproliferative activities on cancer cell lines.
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/3849897