Bile acid sensitivity and in vivo virulence of clinical Clostridium difficile isolates

Clostridium difficile is an anaerobic bacterium that causes diarrheal illnesses. Disease onset is linked with exposure to oral antibiotics and consequent depletion of secondary bile acids. Here we investigate the relationship between in vitro secondary bile acid tolerance and in vivo disease scores...

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Bibliographic Details
Published in:Anaerobe 2016-10, Vol.41, p.32-36
Main Authors: Lewis, Brittany B., Carter, Rebecca A., Pamer, Eric G.
Format: Article
Language:English
Subjects:
acid tolerance
anaerobes
Animals
antibiotics
bacteria
bile acids
Clostridium difficile
Clostridium difficile - drug effects
Clostridium difficile - growth & development
Clostridium difficile - pathogenicity
Enterocolitis, Pseudomembranous - microbiology
Humans
Lithocholic Acid - pharmacology
mice
Mice, Inbred C57BL
Microbial Viability
Secondary bile acid
Spores, Bacterial - drug effects
Spores, Bacterial - growth & development
Spores, Bacterial - pathogenicity
Virulence
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Summary:Clostridium difficile is an anaerobic bacterium that causes diarrheal illnesses. Disease onset is linked with exposure to oral antibiotics and consequent depletion of secondary bile acids. Here we investigate the relationship between in vitro secondary bile acid tolerance and in vivo disease scores of diverse C. difficile strains in mice. •Clostridium difficile isolates collected from patients cause a range of disease in mice.•These isolates exhibit variable tolerance to secondary bile acids in vitro.•Tolerance to lithocholic acid is associated with higher disease scores in mice.
ISSN:1075-9964
1095-8274
DOI:10.1016/j.anaerobe.2016.05.010