Analysis of lipoprotein profiles of healthy cats by gel-permeation high-performance liquid chromatography

Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of animals. We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system to dogs for lipoprotein profil...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2016, Vol.78(8), pp.1277-1281
Main Authors: MIZUTANI, Hisashi, SAKO, Toshinori, OKUDA, Hiroko, ARAI, Nobuaki, KURIYAMA, Koji, MORI, Akihiro, YOSHIMURA, Itaru, KOYAMA, Hidekazu
Format: Article
Language:English
Subjects:
Animals
cat
Cats
Cats - blood
Cats - metabolism
Cholesterol
Cholesterol - blood
Chromatography
Chromatography, High Pressure Liquid - veterinary
Chylomicrons - blood
Density
Electrophoresis
Electrophoresis, Agar Gel - veterinary
Feasibility studies
Gel electrophoresis
High density lipoprotein
High performance liquid chromatography
HPLC
Internal Medicine
lipoprotein profile
Lipoproteins
Lipoproteins (very low density)
Lipoproteins - blood
Lipoproteins, HDL - blood
Lipoproteins, LDL - blood
Lipoproteins, VLDL - blood
Liquid chromatography
Low density lipoprotein
Male
Males
On-line systems
triglyceride
Ultracentrifugation
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Summary:Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of animals. We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system to dogs for lipoprotein profile analysis. We compared the GP-HPLC with DGUC as a method to obtain a feline lipoprotein profile. The lipoprotein profiles showed large and small peaks, which corresponded to high-density lipoprotein (HDL) and low-density lipoprotein (LDL), respectively, whereas very low-density lipoprotein (VLDL) and chylomicron (CM) were only marginally detected. This profile was very similar to that of dogs reported previously. Healthy cats also had a small amount of cholesterol-rich particles distinct from the normal LDL or HDL profile. There was no difference in lipoprotein profiles between the sexes, but males had a significantly larger LDL particle size (P=0.015). This study shows the feasibility of GP-HPLC for obtaining accurate lipoprotein profiles with small sample volumes and provides valuable reference data for healthy cats that should facilitate diagnoses.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.16-0052