Inhibitory effect of leaves extracts of Ocimum basilicum and Ocimum gratissimum on two key enzymes involved in obesity and hypertension in vitro

• Published in: Journal of intercultural ethnopharmacology 2016-01, Vol.5 (4), p.396-402
• Main Authors: Irondi, Emmanuel Anyachukwu, Agboola, Samson Olalekan, Oboh, Ganiyu, Boligon, Aline Augusti
• Format: Article
• Language: English
• Subjects: Original Research
• ISSN: 2146-8397; 2146-8397
• DOI: 10.5455/jice.20160814112756

To evaluate the phenolics composition and inhibitory effect of the leaves extracts of and on two key enzymes (pancreatic lipase [PL] and angiotensin 1-converting enzyme [ACE]) involved in obesity and hypertension . The phenolics (flavonoids and phenolic acids) were quantified using high-performance liquid chromatography coupled with diode array detection. PL and ACE inhibitory effects; DPPH* and ABTS* scavenging activities of the extracts were tested using spectrophotometric methods. had the following major phenolics: Rutin, quercetin, and quercitrin (flavonoids); caffeic, chlorogenic, and gallic acids (phenolic acids); while had the following major phenolics: Rutin, quercitrin, and luteolin (flavonoids); ellagic and chlorogenic acids (phenolic acids). "Extracts of both plants inhibited PL and ACE; scavenged DPPH* in a dose-dependent manner". extract was more potent in inhibiting PL (IC 20.69 µg/mL) and ACE (IC 29.44 µg/mL) than (IC 52.14 µg/mL and IC 64.99 µg/mL, against PL and ACE, respectively). also scavenged DPPH* and ABTS* more than . and leaves could be used as functional foods for the management of obesity and obesity-related hypertension. However, may be more effective than .