Effect of adipose tissue-derived stem cell injection in a rat model of urethral fibrosis

We sought to evaluate the therapeutic effect of adi-pose tissue-derived stem cells (ADSCs) in a rat model of urethral fibrosis. Eighteen (18) male Sprague-Dawley rats (300‒350 g) were divided into three groups: (1) sham (saline injection); (2) urethral fibrosis group (10 μg transforming growth facto...

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Published in:Canadian Urological Association journal 2016-05, Vol.10 (5-6), p.E175-E180
Main Authors: Sangkum, Premsant, Yafi, Faysal A, Kim, Hogyoung, Bouljihad, Mostafa, Ranjan, Manish, Datta, Amrita, Mandava, Sree Harsha, Sikka, Suresh C, Abdel-Mageed, Asim B, Hellstrom, Wayne J G
Format: Article
Language:English
Subjects:
Adipose tissues
Care and treatment
Collagen
Dosage and administration
Fibrosis
Health aspects
Original Research
Stem cells
Transforming growth factors
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Summary:We sought to evaluate the therapeutic effect of adi-pose tissue-derived stem cells (ADSCs) in a rat model of urethral fibrosis. Eighteen (18) male Sprague-Dawley rats (300‒350 g) were divided into three groups: (1) sham (saline injection); (2) urethral fibrosis group (10 μg transforming growth factor beta 1 (TGF-β1) injection); and (3) ADSCs group (10 μg TGF-β1 injection plus 2 × 10 ADSCs). Rat ADSCs were harvested from rat inguinal fat pads. All study animals were euthanized at two weeks after urethral injection. Following euthanasia, rat urethral tissue was harvested for histologic evaluation. Type I and III collagen levels were quantitated by Western blot analysis. TGF-β1 injection induced significant urethral fibrosis and increased collagen type I and III expression (p
ISSN:1911-6470
1920-1214
DOI:10.5489/cuaj.3435