PKIB promotes cell proliferation and the invasion-metastasis cascade through the PI3K/Akt pathway in NSCLC cells

• Published in: Experimental biology and medicine (Maywood, N.J.) N.J.), 2016-11, Vol.241 (17), p.1911-1918
• Main Authors: Dou, Penghui, Zhang, Danfeng, Cheng, Zhuoxin, Zhou, Gang, Zhang, Linyou
• Format: Article
• Language: English
• Subjects: A549 Cells; Blotting, Western; Carcinoma, Non-Small-Cell Lung - pathology; Carcinoma, Non-Small-Cell Lung - physiopathology; Cell Line, Tumor; Cell Proliferation - physiology; Humans; Intracellular Signaling Peptides and Proteins - physiology; Lung Neoplasms - pathology; Lung Neoplasms - physiopathology; Neoplasm Invasiveness - physiopathology; Neoplasm Metastasis - physiopathology; Original Research; Phosphatidylinositol 3-Kinase - metabolism; Proto-Oncogene Proteins c-akt - metabolism; Real-Time Polymerase Chain Reaction; Signal Transduction - physiology
• ISSN: 1535-3702; 1535-3699
• DOI: 10.1177/1535370216655908

Lung cancer is one of the most common malignancies in the world, and non–small cell lung cancer (NSCLC) is a major subtype of lung cancer. Overgrowth of tumor cells usually results from the intensive proliferation of cancer cells, but the mechanisms by which the proliferation of cancer cells are promoted are currently unclear. Thus, it is necessary to determine the vital factors involved in regulating the growth of NSCLC. The MTT assay, BrdU assay, western blots, and migration and invasion assays were used in our study. Here, we found that PKIB (cAMP-dependent protein kinase inhibitor-β), a novel molecular target, was up-regulated in NSCLC tissues compared with the normal tissues adjacent to the tumors. Moreover, overexpression of PKIB promoted cell proliferation and potentiated the invasion and migration in A549 cells, whereas knocking down PKIB gene expression inhibited the proliferation and attenuated the invasive behavior and metastasis in H1299 cells. However, all of these effects of PKIB on cell proliferation and metastasis were reduced by inhibiting the PI3K/Akt pathway. Our results indicate that PKIB promotes cell proliferation and tumorigenesis by activating the PI3K/Akt pathway in NSCLC, implying that this is an important underlying mechanism that affects the progression of NSCLC.