MicroRNA-574-5p promotes metastasis of non-small cell lung cancer by targeting PTPRU

• Published in: Scientific reports 2016-10, Vol.6 (1), p.35714-35714, Article 35714
• Main Authors: Zhou, Rui, Zhou, Xiaoshu, Yin, Zhongyuan, Guo, Jing, Hu, Ting, Jiang, Shun, Liu, Li, Dong, Xiaorong, Zhang, Sheng, Wu, Gang
• Format: Article
• Language: English
• Subjects: 13/109; 14/63; 38/77; 631/67/1612/1350; 631/80/84/2336; 82/80; Adult; Aged; Carcinoma, Non-Small-Cell Lung - physiopathology; Carcinoma, Non-Small-Cell Lung - secondary; Cell Movement - drug effects; Female; Humanities and Social Sciences; Humans; Lung cancer; Male; Metastases; Metastasis; MicroRNAs - metabolism; Middle Aged; miRNA; Molecular modelling; multidisciplinary; Neoplasm Metastasis - physiopathology; Non-small cell lung carcinoma; Phosphorylation; Polymerase chain reaction; Real-Time Polymerase Chain Reaction; Receptor-Like Protein Tyrosine Phosphatases, Class 2 - metabolism; Science; Serum - chemistry; Small cell lung carcinoma; Tyrosine; Wound healing; β-Catenin
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep35714

Dysregulation of microRNAs (miRNAs) has been associated with malignant behavior in a variety of cancers. Our previous study demonstrated that miRNA expression profiles are predictors for patients with advanced non-small cell lung cancer (NSCLC). We also showed that miRNAs are involved in small-cell lung cancer metastasis. Here, we used qRT-PCR to re-analyze our previous microarray results using serum samples from 75 patients with NSCLC. Surprisingly, we found that miR-574-5p and miR-874 were overexpressed in patients with metastatic advanced NSCLC but not in patients with non-metastatic advanced NSCLC. Additionally, miR-574-5p expression was correlated between matched serum and tissue samples from 68 patients. However, these 2 miRNAs are not prognostic factors for NSCLC. Transwell and wound-healing assays showed that miR-574-5p promotes the migration and invasion of NSCLC cells. Furthermore, miR-574-5p enhanced the tyrosine phosphorylation of β-catenin by repressing PTPRU expression in vitro . In conclusion, this study explored the expression of miR-574-5p in clinical samples and its molecular mechanisms in the metastasis of advanced NSCLC.