Microfluidic co-culture system for cancer migratory analysis and anti-metastatic drugs screening

• Published in: Scientific reports 2016-10, Vol.6 (1), p.35544-35544, Article 35544
• Main Authors: Mi, Shengli, Du, Zhichang, Xu, Yuanyuan, Wu, Zhengjie, Qian, Xiang, Zhang, Min, Sun, Wei
• Format: Article
• Language: English
• Subjects: 13/51; 13/62; 14/1; 14/35; 14/63; 38/109; 38/35; 49/62; 631/1647/277; 631/1647/767/70; 631/67/1347; 82/80; Breast Neoplasms - drug therapy; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cancer; Cell adhesion & migration; Cell culture; Cell death; Cell Line, Tumor; Cell migration; Cell Movement; Coculture Techniques - instrumentation; Coculture Techniques - methods; Drug screening; Drug Screening Assays, Antitumor - instrumentation; Drug Screening Assays, Antitumor - methods; Female; Humanities and Social Sciences; Humans; Interleukin 6; Lab-On-A-Chip Devices; Metastases; Metastasis; Microfluidic Analytical Techniques - instrumentation; Microfluidic Analytical Techniques - methods; Microfluidics; multidisciplinary; Neoplasm Metastasis; Paclitaxel; Quantitation; Science; Tamoxifen; Tumors; Velocity
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep35544

Tumour metastasis is an important reason for cancer death, and cancer cell migration is an important step in the process of tumour metastasis. Studying cancer cell migration is of great significance. Here, we present a novel microfluidic co-culture system and establish mild, moderate and severe cancer models by using HMEpiC and MDA-MB–231 cells to study cancer cell migration and anti-cancer drug screening. Using this device, we achieved high cell viability (over 90%) and a stable analysis of the migration ability of cancer cells. We observed that the density of the cancer cells determined the probability of the occurrence of metastatic cells and that the induction of normal cells affected the metastatic velocity of each cancer cell. We verified that the increase in the migration ability of MDA-MB-231 cells co-cultured with HMEpiC cells was relative to the increased secretion of IL-6 and that this was verified by an IL-6 inhibitor assay. This co-culture also led to decreased CK-14 secretion and morphological changes in HMEpiC cells. Finally, significant inhibition of paclitaxel and tamoxifen on cancer migration was observed. Taken together, our microfluidic device could be a useful tool for the quantitation of the migratory capability and anti-metastatic drug screening.