Molecular characterization of the first G24P[14] rotavirus strain detected in humans

• Published in: Infection, genetics and evolution genetics and evolution, 2016-09, Vol.43, p.338-342
• Main Authors: Ward, M. Leanne, Mijatovic-Rustempasic, Slavica, Roy, Sunando, Rungsrisuriyachai, Kunchala, Boom, Julie A., Sahni, Leila C., Baker, Carol J., Rench, Marcia A., Wikswo, Mary E., Payne, Daniel C., Parashar, Umesh D., Bowen, Michael D.
• Format: Article
• Language: English
• Subjects: Animals; Bovine; Cattle; Child, Preschool; G24P; Genome, Viral; Genotype; High-Throughput Nucleotide Sequencing; Humans; Male; Phylogeny; Reassortant Viruses - classification; Reassortant Viruses - genetics; Reassortment; Rotavirus; Rotavirus - classification; Rotavirus - genetics; Rotavirus Infections - virology; Texas; Viral Nonstructural Proteins - genetics
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2016.05.033

Here we report the genome of a novel rotavirus A (RVA) strain detected in a stool sample collected during routine surveillance by the Centers for Disease Control and Prevention's New Vaccine Surveillance Network. The strain, RVA/human-wt/USA/2012741499/2012/G24P[14], has a genomic constellation of G24-P[14]-I2-R2-C2-M2-A3-N2-T9-E2-H3. The VP2, VP3, VP7 and NSP3 genes cluster phylogenetically with bovine strains. The other genes occupy mixed clades containing animal and human strains. Strain RVA/human-wt/USA/2012741499/2012/G24P[14] most likely is the product of interspecies transmission and reassortment events. This is the second report of the G24 genotype and the first report of the G24P[14] genotype combination in humans. •First report of G24P[14] genotype in humans•Second report of G24 genotype overall•Four genes are similar to bovine rotavirus strains.•Other genes resemble animal and human strains.•This strain is likely the product of multiple reassortant events.