Basic methods for fission yeast

The fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its differences from Saccharomyces cerevisiae, the tools and methods for fission yeast are conceptually similar to those used in...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 2006-02, Vol.23 (3), p.173-183
Main Authors: Forsburg, Susan L, Rhind, Nicholas
Format: Article
Language:English
Subjects:
Crosses, Genetic
genetics
microscopy
Molecular Biology - methods
Mycology - methods
Saccharomyces cerevisiae
Schizosaccharomyces - genetics
Schizosaccharomyces - growth & development
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins - genetics
Schizosaccharomyces pombe Proteins - metabolism
Synchrony
transformation
Transformation, Genetic
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Summary:The fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its differences from Saccharomyces cerevisiae, the tools and methods for fission yeast are conceptually similar to those used in budding yeast. Here, we present basic methods sufficient for a beginner in this system to carry out most required manipulations for genetic analysis or molecular biology. Copyright © 2006 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.1347