Comparison of micro-CT post-processing methods for evaluating the trabecular bone volume fraction in a rat ACL-transection model

Abstract Trabecular bone volume fraction assessments are likely sensitive to the analysis method and selection of the region of interest. Currently, there are several methods for selecting the region of interest to analyze trabecular bone in animal models of post-traumatic osteoarthritis. The object...

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Bibliographic Details
Published in:Journal of biomechanics 2016-10, Vol.49 (14), p.3559-3563
Main Authors: Chin, K.E, Karamchedu, N.P, Patel, T.K, Badger, G.J, Akelman, M.R, Moore, D.C, Proffen, B.L, Murray, M.M, Fleming, B.C
Format: Article
Language:English
Subjects:
Animals
Anterior Cruciate Ligament - diagnostic imaging
Anterior Cruciate Ligament - surgery
Assessments
Biomedical materials
Bone
Bones
Cancellous Bone - diagnostic imaging
Cancellous Bone - physiology
Compartments
Core loss
Fractions
Hindlimb - diagnostic imaging
Image Processing, Computer-Assisted - methods
Image segmentation
Knee
Knees
Ligament
Methods
Micro-computed tomography
Osteoarthritis
Physical Medicine and Rehabilitation
Rats, Inbred Lew
Scanners
Sensitivity analysis
Studies
Tibia - diagnostic imaging
Tibia - physiology
Tomography
Variance analysis
Volume fraction
X-Ray Microtomography
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Summary:Abstract Trabecular bone volume fraction assessments are likely sensitive to the analysis method and selection of the region of interest. Currently, there are several methods for selecting the region of interest to analyze trabecular bone in animal models of post-traumatic osteoarthritis. The objective of this study was to compare three published methods for determining the trabecular bone volume fraction of the medial tibial epiphyses in ACL transected and contralateral ACL intact knees. Micro-computed tomography images of both knees were obtained five weeks post-operatively and evaluated using three methods: (1) the Whole Compartment Method that captured the entire medial compartment, (2) the centrally located Single Core Method, and (3) the Triplet Core Method that averaged focal locations in the anterior, central, and posterior regions. The Whole Compartment Method detected significant bone loss in the ACL transected knee compared to the ACL intact knee ( p
ISSN:0021-9290
1873-2380
DOI:10.1016/j.jbiomech.2016.08.024