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Mechanism of dexamethasone suppression of brain tumor-associated vascular permeability in rats. Involvement of the glucocorticoid receptor and vascular permeability factor

Brain tumor-associated cerebral edema arises because tumor capillaries lack normal blood-brain barrier function; vascular permeability factor (VPF, also known as vascular endothelial growth factor, VEGF) is a likely mediator of this phenomenon. Clinically, dexamethasone reduces brain tumor-associate...

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Published in:The Journal of clinical investigation 1996-09, Vol.98 (6), p.1400-1408
Main Authors: Heiss, J D, Papavassiliou, E, Merrill, M J, Nieman, L, Knightly, J J, Walbridge, S, Edwards, N A, Oldfield, E H
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container_title The Journal of clinical investigation
container_volume 98
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Papavassiliou, E
Merrill, M J
Nieman, L
Knightly, J J
Walbridge, S
Edwards, N A
Oldfield, E H
description Brain tumor-associated cerebral edema arises because tumor capillaries lack normal blood-brain barrier function; vascular permeability factor (VPF, also known as vascular endothelial growth factor, VEGF) is a likely mediator of this phenomenon. Clinically, dexamethasone reduces brain tumor-associated vascular permeability through poorly understood mechanisms. Our goals were to determine if suppression of permeability by dexamethasone might involve inhibition of VPF action or expression, and if dexamethasone effects in this setting are mediated by the glucocorticoid receptor (GR). In two rat models of permeability (peripheral vascular permeability induced by intradermal injection of 9L glioma cell-conditioned medium or purified VPF, and intracerebral vascular permeability induced by implanted 9L glioma), dexamethasone suppressed permeability in a dose-dependent manner. Since 80% of the permeability-inducing activity in 9L-conditioned medium was removed by anti-VPF antibodies, we examined dexamethasone effects of VPF expression in 9L cells. Dexamethasone inhibited FCS- and PDGF-dependent induction of VPF expression. At all levels (intradermal, intracranial, and cell culture), dexamethasone effects were reversed by the GR antagonist mifepristone (RU486). Dexamethasone may decrease brain tumor-associated vascular permeability by two GR-dependent mechanisms: reduction of the response of the vasculature to tumor-derived permeability factors (including VPF), and reduction of VPF expression by tumor cells.
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subjects Animals
Antibodies, Blocking - immunology
Blotting, Northern
Brain Neoplasms - metabolism
Brain Neoplasms - physiopathology
Capillary Permeability
Cells, Cultured
Culture Media, Conditioned
Dexamethasone - pharmacology
Dose-Response Relationship, Drug
Endothelial Growth Factors - biosynthesis
Endothelial Growth Factors - immunology
Endothelial Growth Factors - pharmacology
Glioma - metabolism
Glioma - physiopathology
Lymphokines - biosynthesis
Lymphokines - immunology
Lymphokines - pharmacology
Mifepristone - pharmacology
Platelet-Derived Growth Factor - metabolism
Rats
Receptors, Glucocorticoid - metabolism
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
title Mechanism of dexamethasone suppression of brain tumor-associated vascular permeability in rats. Involvement of the glucocorticoid receptor and vascular permeability factor
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