M-DNA/Transition Metal Dichalcogenide Hybrid Structure-based Bio-FET sensor with Ultra-high Sensitivity

Here, we report a high performance biosensor based on ( i ) a Cu 2+ -DNA/MoS 2 hybrid structure and ( ii ) a field effect transistor, which we refer to as a bio-FET, presenting a high sensitivity of 1.7 × 10 3  A/A. This high sensitivity was achieved by using a DNA nanostructure with copper ions (Cu...

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Published in:Scientific reports 2016-10, Vol.6 (1), p.35733, Article 35733
Main Authors: Park, Hyung-Youl, Dugasani, Sreekantha Reddy, Kang, Dong-Ho, Yoo, Gwangwe, Kim, Jinok, Gnapareddy, Bramaramba, Jeon, Jaeho, Kim, Minwoo, Song, Young Jae, Lee, Sungjoo, Heo, Jonggon, Jeon, Young Jin, Park, Sung Ha, Park, Jin-Hong
Format: Article
Language:English
Subjects:
639/166/987
639/925/927/59
Biosensors
Copper
Deoxyribonucleic acid
DNA
DNA structure
Doxorubicin
Humanities and Social Sciences
multidisciplinary
Polarity
Raman spectroscopy
Science
Spectroscopy
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Summary:Here, we report a high performance biosensor based on ( i ) a Cu 2+ -DNA/MoS 2 hybrid structure and ( ii ) a field effect transistor, which we refer to as a bio-FET, presenting a high sensitivity of 1.7 × 10 3  A/A. This high sensitivity was achieved by using a DNA nanostructure with copper ions (Cu 2+ ) that induced a positive polarity in the DNA (receptor). This strategy improved the detecting ability for doxorubicin-like molecules (target) that have a negative polarity. Very short distance between the biomolecules and the sensor surface was obtained without using a dielectric layer, contributing to the high sensitivity. We first investigated the effect of doxorubicin on DNA/MoS 2 and Cu 2+ -DNA/MoS 2 nanostructures using Raman spectroscopy and Kelvin force probe microscopy. Then, we analyzed the sensing mechanism and performance in DNA/MoS 2 - and Cu 2+ -DNA/MoS 2 -based bio-FETs by electrical measurements (I D -V G at various V D ) for various concentrations of doxorubicin. Finally, successful operation of the Cu 2+ -DNA/MoS 2 bio-FET was demonstrated for six cycles (each cycle consisted of four steps: 2 preparation steps, a sensing step, and an erasing step) with different doxorubicin concentrations. The bio-FET showed excellent reusability, which has not been achieved previously in 2D biosensors.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep35733