miR-940 Suppresses Tumor Cell Invasion and Migration via Regulation of CXCR2 in Hepatocellular Carcinoma

Aim. To investigate the expression of miR-940 in the hepatocellular carcinoma (HCC) and its impact on function and biological mechanism in the HCC cells. Methods. Quantitative RT-PCR analysis was used to quantify miR-940 expression in 46 cases of tissues and cells. Transfection of HCC cell lines was...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-8
Main Authors: Li, Xiangcheng, Huo, Liqun, Ji, Guwei, Wang, Xing, Yang, Renjie, Zhang, Yaodong, Ding, Dong, Shao, Zicheng
Format: Article
Language:English
Subjects:
Analysis
Apoptosis
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - genetics
Biomedical research
Cancer therapies
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - mortality
Cell Line, Tumor
Cell Movement
Chemokines
Cytokines
Female
Gene Expression Regulation, Neoplastic
Hepatoma
Hospitals
Humans
Inflammation
Liver cancer
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Liver Neoplasms - mortality
Male
Metastasis
MicroRNAs - biosynthesis
MicroRNAs - genetics
Neoplasm Invasiveness
Neoplasm Proteins - biosynthesis
Neoplasm Proteins - genetics
Prostate cancer
Receptors, Interleukin-8B - biosynthesis
Receptors, Interleukin-8B - genetics
RNA, Neoplasm - biosynthesis
RNA, Neoplasm - genetics
Roles
Studies
Tumors
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Summary:Aim. To investigate the expression of miR-940 in the hepatocellular carcinoma (HCC) and its impact on function and biological mechanism in the HCC cells. Methods. Quantitative RT-PCR analysis was used to quantify miR-940 expression in 46 cases of tissues and cells. Transfection of HCC cell lines was performed by miR-940 mimics; the abilities of invasion and migration were assessed through Transwell array. Western blot represents the alteration in expression of CXCR2 by miR-940 mimics. Results. miR-940 expression was decreased significantly in the HCC tissues and the relevant cell lines. miR-940 upregulation suppressed the invasion and migration of HCC cells in vitro. Furthermore, the CXCR2 was downregulated to suppress invasion and migration after miR-940 mimics. Moreover, decreased miR-940 expression was negatively correlated with Edmondson grade (P=0.008), tumor microsatellite or multiple tumors (P=0.04), vascular invasion (P=0.035), and recurrence and metastasis (P=0.038). Kaplan-Meier analysis demonstrated that decreased miR-940 expression contributed to poor overall survival (P
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/7618342