Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on Biomarkers

Senescence of cardiac myocytes is frequently associated with heart diseases. To analyze senescence in cardiac myocytes, a number of biomarkers have been isolated. However, due to the complex nature of senescence, multiple markers are required for a single assay to accurately depict complex physiolog...

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Bibliographic Details
Published in:BioMed research international 2016-01, Vol.2016 (2016), p.1-9
Main Authors: Lee, Jong-Hyun, Jin, Suk-Won, Jung, Minhyun, Kang, Giseok, Park, Chang-Ju, Kim, Hyeon Woo, Yun, Joho, Park, Yangkyu, Cha, Jung-Joon, Pak, Boryeong
Format: Article
Language:English
Subjects:
Animals
Biological markers
Biomarkers - metabolism
Cell Membrane - metabolism
Cell Membrane - physiology
Cellular Senescence - physiology
Cytoplasm - metabolism
Cytoplasm - physiology
Danio rerio
Dielectric Spectroscopy - methods
Electric Impedance
Electric properties
Heart diseases
Microelectrodes
Myocytes, Cardiac - metabolism
Myocytes, Cardiac - physiology
Science
Studies
Zebrafish
Zebrafish - metabolism
Zebrafish - physiology
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Summary:Senescence of cardiac myocytes is frequently associated with heart diseases. To analyze senescence in cardiac myocytes, a number of biomarkers have been isolated. However, due to the complex nature of senescence, multiple markers are required for a single assay to accurately depict complex physiological changes associated with senescence. In single cells, changes in both cytoplasm and cell membrane during senescence can affect the changes in electrical impedance. Based on this phenomenon, we developed MEDoS, a novel microelectrochemical impedance spectroscopy for diagnosis of senescence, which allows us to precisely measure quantitative changes in electrical properties of aging cells. Using cardiac myocytes isolated from 3-, 6-, and 18-month-old isogenic zebrafish, we examined the efficacy of MEDoS and showed that MEDoS can identify discernible changes in electrical impedance. Taken together, our data demonstrated that electrical impedance in cells at different ages is distinct with quantitative values; these results were comparable with previously reported ones. Therefore, we propose that MEDoS be used as a new biomarker-independent methodology to obtain quantitative data on the biological senescence status of individual cells.
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/8484217