Oocyte aging-induced Neuronatin (NNAT) hypermethylation affects oocyte quality by impairing glucose transport in porcine

DNA methylation plays important roles in regulating many physiological behaviors; however, few studies were focused on the changes of DNA methylation during oocyte aging. Early studies showed that some imprinted genes’ DNA methylation had been changed in aged mouse oocytes. In this study, we used po...

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Bibliographic Details
Published in:Scientific reports 2016-10, Vol.6 (1), p.36008-36008, Article 36008
Main Authors: Gao, Ying-Ying, Chen, Li, Wang, Tao, Nie, Zheng-Wen, Zhang, Xia, Miao, Yi-Liang
Format: Article
Language:English
Subjects:
13/1
38/32
631/80/128
631/80/641/1633
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Aging
Deoxyribonucleic acid
DNA
DNA methylation
Glucose
Glucose transport
Humanities and Social Sciences
multidisciplinary
Oocytes
Science
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Summary:DNA methylation plays important roles in regulating many physiological behaviors; however, few studies were focused on the changes of DNA methylation during oocyte aging. Early studies showed that some imprinted genes’ DNA methylation had been changed in aged mouse oocytes. In this study, we used porcine oocytes to test the hypothesis that oocyte aging would alter DNA methylation pattern of genes and disturb their expression in age oocytes, which affected the developmental potential of oocytes. We compared several different types of genes and found that the expression and DNA methylation of Neuronatin ( NNAT ) were disturbed in aged oocytes significantly. Additional experiments demonstrated that glucose transport was impaired in aged oocytes and injection of NNAT antibody into fresh oocytes led to the same effects on glucose transport. These results suggest that the expression of NNAT was declined by elevating DNA methylation, which affected oocyte quality by decreasing the ability of glucose transport in aged oocytes.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep36008