Simple Plex™: A Novel Multi-Analyte, Automated Microfluidic Immunoassay Platform for the Detection of Human and Mouse Cytokines and Chemokines

Problem Quantitative measurement of proteins in bodily fluids or cellular preparations is critical for the evaluation of biomarkers or the study of complex cellular processes. While immunoassays are the most common quantitative approach used so far, they are not practical for the evaluation of multi...

Full description

Saved in:
Bibliographic Details
Published in:American journal of reproductive immunology (1989) 2016-06, Vol.75 (6), p.678-693
Main Authors: Aldo, Paulomi, Marusov, Gregory, Svancara, Danielle, David, James, Mor, Gil
Format: Article
Language:English
Subjects:
Animals
Automation
Automation, Laboratory
Chemokines
Chemokines - blood
Cytokines
Cytokines - blood
Enzyme-Linked Immunosorbent Assay
Humans
Immunoassay
Lab-On-A-Chip Devices
Mice
Microfluidics - instrumentation
Microfluidics - methods
Miniaturization
multiplex
Observer Variation
Proteins
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Simple Plex
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Problem Quantitative measurement of proteins in bodily fluids or cellular preparations is critical for the evaluation of biomarkers or the study of complex cellular processes. While immunoassays are the most common quantitative approach used so far, they are not practical for the evaluation of multiple proteins. Microfluidic technology allows a fine spatial control in immobilizing proteins and biomolecules inside microchannels, eliminating cross‐reactivity between competing analytes, and allowing rapid and sensitive detection of targeted antigens for multiple applications. We report the characterization and validation of the Simple Plex™ platform for the detection and quantification of cytokines and chemokines from human and mouse samples. Method Cytokine and chemokine expression levels were determined using Simple Plex cartridges from ProteinSimple. Serum samples were obtained from the Yale Biorepository. Results Our data demonstrate an excellent correlation between the results obtained with Simple Plex and conventional immunoassays such as ELISA and Luminex. Conclusion We describe the characterization and validation of Simple Plex, a novel multi‐analyte, automated microfluidic platform that allows the evaluation of cytokines and chemokines from human and mice biological samples. Simple Plex showed significant advantages over traditional approaches in terms of low sample volume requirements, sensitivity and dynamic range, coefficient of variation, and reproducibility.
ISSN:1046-7408
1600-0897
DOI:10.1111/aji.12512